Evidence map›Paper›PMID 26438643›Full record

ArticleJournal of assisted reproduction and genetics2015

Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts.

P Tulay, R P Naja, O Cascales-Roman, A Doshi, P Serhal, S B SenGupta

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

P TulayFaculty of Medicine, Medical Genetics Department, Near East University, Yakin Dogu Bulvari, Nicosia, Cyprus. pintulay@gmail.com.
R P NajaUCL Centre for PGD, Institute for Women's Health, University College London, London, UK.
O Cascales-RomanUCL Centre for PGD, Institute for Women's Health, University College London, London, UK.
A DoshiThe Centre for Reproductive and Genetic Health, University College Hospital, The New Wing Eastman Dental Hospital, London, UK.
P SerhalThe Centre for Reproductive and Genetic Health, University College Hospital, The New Wing Eastman Dental Hospital, London, UK.
S B SenGuptaUCL Centre for PGD, Institute for Women's Health, University College London, London, UK.
University College London · GBEastman Dental Hospital · GBNear East University · CY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe aim of the study is to investigate the regulation of DNA repair genes by microRNAs (miRNAs). miRNAs are short non-coding RNAs that regulate transcriptional and post-transcriptional gene silencing. Several miRNAs that are expressed during preimplantation embryo development have been shown or are predicted to target genes that regulate cell cycle checkpoints and DNA repair in response to DNA damage.

methodsThis study compares the expression level of 20 miRNAs and 9 target transcripts involved in DNA repair. The statistical significance of differential miRNA expression between oocytes and blastocysts was determined by t test analysis using the GraphPad Prism v6 software. The possible regulatory roles of miRNAs on their target messenger RNAs (mRNAs) were analysed using a Pearson correlation test.

resultsThis study shows for the first time that several miRNAs are expressed in human oocytes and blastocysts that target key genes involved in DNA repair and cell cycle checkpoints. Blastocysts exhibited statistically significant lower expression levels for the majority of miRNAs compared to oocytes (p < 0.05). Correlation analyses showed that there was both inverse and direct association between miRNAs and their target mRNAs.

conclusionsmiRNAs target many mRNAs including ones involved in DNA repair mechanisms. This study suggests that miRNAs and their target mRNAs involved in DNA repair are expressed in preimplantation embryos. Similar to the miRNAs expressed in adult tissues, these miRNAs seem to have regulatory roles on their target DNA repair mRNAs during preimplantation embryo development.

Indexed as

AdultBlastocystDNA RepairGene Expression Regulation, DevelopmentalHumansMicroRNAsOocytesRNA, MessengerMicroRNAsRNA, MessengerDNA repairExpressionHuman oocyte and blastocystMicroRNAPreimplantation embryo development

Identifiers

PMID26438643
PMCPMC4681740
OpenAlexW1851524421

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.