Evidence map›Paper›PMID 26438601›Full record

ArticleMolecular and cellular biology2015

The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop.

Wayne O Miles, Julie M J Lepesant, Jessie Bourdeaux, Manuela Texier, Marc A Kerenyi, Makoto Nakakido, Ryuji Hamamoto, Stuart H Orkin, Nicholas J Dyson, Luisa Di Stefano

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Wayne O MilesLaboratory of Molecular Oncology, Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts, USA.
Julie M J LepesantLBCMCP, Université Paul Sabatier, CNRS, Toulouse, France.
Jessie BourdeauxLBCMCP, Université Paul Sabatier, CNRS, Toulouse, France.
Manuela TexierLBCMCP, Université Paul Sabatier, CNRS, Toulouse, France.
Marc A KerenyiBoston Children's Hospital and Dana-Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA.
Makoto NakakidoDepartment of Medicine, University of Chicago, Chicago, Illinois, USA.
Ryuji HamamotoDepartment of Medicine, University of Chicago, Chicago, Illinois, USA.
Stuart H OrkinBoston Children's Hospital and Dana-Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA.
Nicholas J DysonLaboratory of Molecular Oncology, Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts, USA.
Luisa Di StefanoLBCMCP, Université Paul Sabatier, CNRS, Toulouse, France luisa.di-stefano@univ-tlse3.fr.
Harvard University · USLaboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération · FRMassachusetts General Hospital · USUniversity of Chicago · USCentre National de la Recherche Scientifique · FRUniversité Toulouse III - Paul Sabatier · FR

Funding

Drosophila Transgenic RNAi Resource ProjectR01GM084947 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2008 to 2019
$9.8M
MUTAGENESIS OF THE PRB POCKET DOMAINR01CA064402 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI DYSON, NICHOLAS J · 1994 to 2015
$4.1M
Dissection and manipulation of RB functionR01CA163698 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI DYSON, NICHOLAS J · 2012 to 2016
$1.8M
NCI NIH HHS R01 CA064402NCI NIH HHS R01 CA163698NCI NIH HHS R01 CA64402NIGMS NIH HHS R01 GM084947NIGMS NIH HHS R01-GM084947
6 · The paper itself

Abstract

The lysine (K)-specific demethylase (LSD1) family of histone demethylases regulates chromatin structure and the transcriptional potential of genes. LSD1 is frequently deregulated in tumors, and depletion of LSD1 family members causes developmental defects. Here, we report that reductions in the expression of the Pumilio (PUM) translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. We show that the PUM complex is a target of LSD1 regulation in fly and mammalian cells and that its expression is inversely correlated with LSD1 levels in human bladder carcinoma. Unexpectedly, we find that PUM posttranscriptionally regulates LSD1 family protein levels in flies and human cells, indicating the existence of feedback loops between the LSD1 family and the PUM complex. Our results highlight a new posttranscriptional mechanism regulating LSD1 activity and suggest that the feedback loop between the LSD1 family and the PUM complex may be functionally important during development and in human malignancies.

Indexed as

Feedback, PhysiologicalAnimalsCell Line, TumorDNA-Binding ProteinsDrosophilaDrosophila ProteinsHeLa CellsHistone DemethylasesHumansMCF-7 CellsMiceOxidoreductases, N-DemethylatingRNA-Binding ProteinsRNA InterferenceRNA, MessengerRNA Processing, Post-Transcriptionalbrat protein, DrosophilaDNA-Binding ProteinsDrosophila ProteinsHistone DemethylasesLsd-1 protein, Drosophilanos protein, DrosophilaOxidoreductases, N-Demethylatingpum protein, DrosophilaRNA-Binding ProteinsRNA, MessengerRNA, Small Interfering

Identifiers

PMID26438601
PMCPMC4648826
OpenAlexW2174325243

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.