ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2016
Pharmacological identification of cholinergic receptor subtypes on Drosophila melanogaster larval heart.
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Genetic architecture of basal heart rate and its modulation by dietary salt in Drosophila melanogaster.PLoS genetics · 2026Article
- A mechanosensitive vascular niche forProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- Smoking flies: testing the effect of tobacco cigarettes on heart function ofBiology open · 2021Article
- Three-dimensional analysis of the heart function and effect cholinergic agonists in the cockroach Gromphadorhina portentosa.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2020Article
- The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila.Invertebrate neuroscience : IN · 2020Article
- Impedance Measures and a Mounting Technique for Drosophila: Larval Movements, Heart Rate, Imaging, and Electrophysiology.Methods and protocols · 2020Article
- Acetylcholine and Its Receptors in Honeybees: Involvement in Development and Impairments by Neonicotinoids.Insects · 2019Review
- Effects of inhibiting mTOR with rapamycin on behavior, development, neuromuscular physiology and cardiac function in larvalBiology open · 2019Article
- The Effects of Chloride Flux onMethods and protocols · 2019Article
- Development and evolution of the metazoan heart.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Cholinergic Agonists and Antagonists Have an Effect on the Metabolism of the BeetleMolecules (Basel, Switzerland) · 2018Article
- Sources of CaJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Using optogenetics to assess neuroendocrine modulation of heart rate in Drosophila melanogaster larvae.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2017Article
- Modulatory effects on Drosophila larva hearts: room temperature, acute and chronic cold stress.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- Optogenetic stimulation of Drosophila heart rate at different temperatures and Ca2+ concentrations.Physiological reports · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila melanogaster heart is a popular model in which to study cardiac physiology and development. Progress has been made in understanding the role of endogenous compounds in regulating cardiac function in this model. It is well characterized that common neurotransmitters act on many peripheral and non-neuronal tissues as they flow through the hemolymph of insects. Many of these neuromodulators, including acetylcholine (ACh), have been shown to act directly on the D. melanogaster larval heart. ACh is a primary neurotransmitter in the central nervous system (CNS) of vertebrates and at the neuromuscular junctions on skeletal and cardiac tissue. In insects, ACh is the primary excitatory neurotransmitter of sensory neurons and is also prominent in the CNS. A full understanding regarding the regulation of the Drosophila cardiac physiology by the cholinergic system remains poorly understood. Here we use semi-intact D. melanogaster larvae to study the pharmacological profile of cholinergic receptor subtypes, nicotinic acetylcholine receptors (nAChRs) and muscarinic acetylcholine receptors (mAChRs), in modulating heart rate (HR). Cholinergic receptor agonists, nicotine and muscarine both increase HR, while nAChR agonist clothianidin exhibits no significant effect when exposed to an open preparation at concentrations as low as 100 nM. In addition, both nAChR and mAChR antagonists increase HR as well but also display capabilities of blocking agonist actions. These results provide evidence that both of these receptor subtypes display functional significance in regulating the larval heart's pacemaker activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.