ArticleParasites & vectors2015
Development of a generic Ehrlichia FRET-qPCR and investigation of ehrlichioses in domestic ruminants on five Caribbean islands.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Efficacy of Minocycline in Naturally Occurring Nonacute Ehrlichia canis Infection in Dogs.Journal of veterinary internal medicine · 2018Trial
- Clinical insights into Candidatus Mycoplasma haemominutum-associated anaemia in naturally infected cats: flea-associated risk and paradoxical clinicopathological findings.Irish veterinary journal · 2026Article
- Article
- Molecular Characterization of Tick-borne Pathogens in Bactrian Camels and Ticks from Gansu Province, China.Acta parasitologica · 2024Article
- Whole Genome Sequencing and Comparative Analysis of the FirstMicroorganisms · 2024Article
- Epidemiology of Ticks and Tick-Borne Pathogens in Domestic Ruminants across Southern African Development Community (SADC) Region from 1980 until 2021: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2022Review
- Molecular discrimination and genetic diversity of three common tick-borne pathogens in dogs in Thailand.Parasitology · 2022Article
- Ticks and Tick-Borne Diseases in Central America and the Caribbean: A One Health Perspective.Pathogens (Basel, Switzerland) · 2021Review
- Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from the Caribbean.Ticks and tick-borne diseases · 2021Article
- Development of a Multiplex PCR and Magnetic DNA Capture Assay for Detecting Six Species Pathogens of the GeneraPathogens (Basel, Switzerland) · 2021Article
- Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis.BMC genomics · 2021Article
- First molecular detection ofOpen veterinary journal · 2020Article
- Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh Province, Saudi Arabia.Parasites & vectors · 2020Article
- Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal.Parasites & vectors · 2019Article
- Molecular characterization of Anaplasma and Ehrlichia in ixodid ticks and reservoir hosts from Palestine: a pilot survey.Veterinary medicine and science · 2019Article
- Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens.Parasites & vectors · 2018Article
- Ecological preferences and seasonal dynamics of ticks (Acari: Ixodidae) on and off bovine hosts in the Eastern Cape Province, South Africa.Experimental & applied acarology · 2018Article
- Complete Genome Sequence of Ehrlichia canis Strain YZ-1, Isolated from a Beagle with Fever and Thrombocytopenia.Genome announcements · 2018Article
- Survey of vector-borne agents in feral cats and first report of Babesia gibsoni in cats on St Kitts, West Indies.BMC veterinary research · 2017Article
- Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Ehrlichia are obligate intracellular Gram-negative tick-borne bacteria that are important human and animal pathogens. There is a need for assays to rapidly and reliably detect and differentiate the five generally recognized species into groups in a single reaction: E. canis, E. chaffeensis, E. ewingii, E. muris and E. ruminantium.
methodsWe developed primers and probes against the 16S rRNA gene to enable us to reliably detect the five major Ehrlichia spp. in a single FRET-qPCR. We tested the Ehrlichia FRET-qPCR on reference strains and on DNA from the blood of domestic ruminants from five Caribbean islands. The Ehrlichia present were determined using melting point analysis and by sequencing the Ehrlichia FRET-qPCR products as well as those of a nested PCR against the citrate synthase gene (gltA).
resultsOur Ehrlichia FRET-qPCR was negative for the closely related Anaplasma marginale and A. phagocytophilum but gave positive reactions with reference strains of the most generally recognized species and with other less characterized Ehrlichia of domestic ruminants, mainly E. ovina, the Panola Mountain Ehrlichia, and Ehrlichia sp. BOV2010. Melting point analysis revealed 4 distinct groups: E. ruminantium (T m ~55.8 °C); E. chaffeensis and E. ewingii (T m ~57.7 °C); E. canis, E. muris, E. ovina and Ehrlichia sp. BOV 2010 (T m ~62.0 °C); and the Panola Mountain Ehrlichia (T m ~65.5 °C). The detection limit of the FRET-qPCR was ~ 5 gene copies in a reaction and the sequences of the FRET-qPCR products were as expected. With DNA from domestic ruminants from the Caribbean we found 12.2 % (134/1,101) positive: cattle (76/385; 19.7 %), sheep (45/340; 13.2 %) and goats (13/376; 3.5 %). Melting point analysis and sequencing of the FRET-qPCR and nested PCR gltA products showed the Ehrlichia we detected were E. canis or very closely related organisms.
conclusionsIn a single reaction, our Ehrlichia FRET-qPCR can detect the Ehrlichia spp. we studied and differentiate them into four groups. Domestic ruminants in the Caribbean are not uncommonly exposed to Ehrlichia, possibly E. canis or very closely related organisms.
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