ArticleMicrobial cell factories2015
Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- The Distribution of Several Genomic Virulence Determinants Does Not Corroborate the Established Serotyping Classification ofInternational journal of molecular sciences · 2021Article
- Enhancing the insecticidal activity of new Bacillus thuringiensis X023 by copper ions.Microbial cell factories · 2020Article
- Role ofFrontiers in microbiology · 2019Article
- Comparative Analysis of Genomics and Proteomics in the New Isolated Bacillus thuringiensis X022 Revealed the Metabolic Regulation Mechanism of Carbon Flux Following Cu(2+) Treatment.Frontiers in microbiology · 2016Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However, the molecular mechanisms of the effects caused by mineral elements have yet to be reported.
resultsThe ICP are mainly composed of Cry1Ca, Cry1Ac, and Cry1Da, which have molecular weights of about 130 kDa. ICP production was most efficient when Cu(2+) was added at concentrations ranging from 10(-6) to 10(-4) mol/L at an initial pH of 8.0. Addition of Cu(2+) also evidently increased the toxicity of fermentation broth to Spodoptera exigua and Helicoverpa armigera. After analyzing changes in proteome and fermentation parameters caused by Cu(2+) addition, we propose that Cu(2+) increases PhaR expression and consequently changes the carbon flow. More carbon sources was used to produce intracellular poly-β-hydroxybutyrate (PHB). Increases in PHB as a storage material bring about increases of ICP production.
conclusionsBacillus thuringiensis X022 mainly expresses Cry1Ca, Cry1Ac, and Cry1Da. Cu(2+) increases the expression of Cry1Da, Cry1Ca, and also enhances the toxicity of fermentation broth to S. exigua and H. armigera.
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