ArticleMolecular genetics and genomics : MGG2016
Selection of reference genes for analysis of stress-responsive genes after challenge with viruses and temperature changes in the silkworm Bombyx mori.
Article in Molecular genetics and genomics : MGG, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 76 citations in OpenAlex.
- The role of BmsPLA2-4 in tissue defense against pathogens in silkworm, Bombyx mori.Insect science · 2026Article
- The Weak Worker Hypothesis: a new framework for understanding division of labour in social insects.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Transcriptomics and Metabolomics Combined to Analyze the Response Mechanism of Silkworm Eggs to High-Temperature Stress.Insects · 2025Article
- Evaluation of stable reference genes for RT-qPCR analysis in Antheraea assamensis (Lepidoptera: Saturniidae) during pathogenic infections.Molecular biology reports · 2025Article
- Transgenic overexpression of UDP glycosyltransferase gene UGT41A3 induces resistance to nucleopolyhedrovirus in Bombyx mori.Transgenic research · 2024Article
- The Role ofInsects · 2024Article
- Downregulation ofFrontiers in microbiology · 2024Article
- Identification of specific reference gene for normalization of RT-qPCR data in rhythmic gene expression studies of the effect of developmental hormone antagonist in postembryonic development inFrontiers in insect science · 2024Article
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- Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data.BMC genomics · 2023Article
- Identification and Validation of Reliable Reference Genes for Gene Expression Studies inGenes · 2022Article
- Identification and Characterization of BmNPV m6A Sites and Their Possible Roles During Viral Infection.Frontiers in immunology · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in physiology · 2022Article
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- Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis inFrontiers in physiology · 2021Article
- Precocious Metamorphosis of Silkworm Larvae Infected by BmNPV in the Latter Half of the Fifth Instar.Frontiers in physiology · 2021Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses and high temperature (HT) are the primary threats to silkworms. Changes in the expression of stress-response genes can be measured using quantitative polymerase chain reaction (qPCR) after exposure to viruses or HT. However, appropriate reference genes (RGs) for qPCR data normalization have not been established in this organism. In this study, we summarized the RGs used in the previous silkworm studies after infection with Bombyx mori nucleopolyhedrovirus (BmNPV), B. mori cytoplasmic polyhedrosis virus (BmCPV), or B. mori densovirus (BmDNV) or after HT treatment. The expression levels of these RGs were extracted from silkworm transcriptome data to screen for candidate RGs that were unaffected by the experimental conditions. Actin-1 (A1), actin-3 (A3), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and translation initiation factor 4a (TIF-4A) were selected for further qPCR verification. The results of RNA-seq and qPCR showed that GAPDH and TIF-4A were suitable RGs after BmNPV challenge or HT stress, whereas TIF-4A was an appropriate RG for BmCPV or BmDNV-Z challenge in silkworms. These results suggested that TIF-4A may be the most appropriate RG for gene expression analysis after challenge with viruses or HT in silkworms.
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