ArticleDevelopmental neurobiology2016
Intraspinal serotonergic neurons consist of two, temporally distinct populations in developing zebrafish.
Article in Developmental neurobiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Serotonergic Control of Spine Morphology During Growth in Zebrafish.bioRxiv : the preprint server for biology · 2026Article
- Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity.Nature communications · 2026Article
- Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity.bioRxiv : the preprint server for biology · 2025Article
- An In Vivo Drug Screen Reveals That Sirtuin 2 Activity Promotes Spinal Cord Neurogenesis in Developing Zebrafish.Biomolecules · 2025Article
- Organization of Serotonergic Cell Populations in the Brain and Spinal Cord of the Short-Lived African Turquoise Killifish.Biology · 2025Article
- An in vivo drug screen in zebrafish reveals that cyclooxygenase 2-derived prostaglandin DCell proliferation · 2024Article
- Roles of cerebrospinal fluid-contacting neurons as potential neural stem cells in the repair and regeneration of spinal cord injuries.Frontiers in cell and developmental biology · 2024Review
- Cerebrospinal fluid-contacting neurons: multimodal cells with diverse roles in the CNS.Nature reviews. Neuroscience · 2023Review
- Article
- Repetitive optogenetic stimulation of glutamatergic neurons: An alternative to NMDA treatment for generating locomotor activity in spinalized zebrafish larvae.Physiological reports · 2021Article
- The serotonin reuptake blocker citalopram destabilizes fictive locomotor activity in salamander axial circuits through 5-HTJournal of neurophysiology · 2020Article
- Serotonergic Modulation of Locomotor Activity From Basal Vertebrates to Mammals.Frontiers in neural circuits · 2020Review
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- Article
- Fgf3 is crucial for the generation of monoaminergic cerebrospinal fluid contacting cells in zebrafish.Biology open · 2019Article
- Intraspinal serotonergic signaling suppresses locomotor activity in larval zebrafish.Developmental neurobiology · 2018Article
- Complementary expression of calcium binding proteins delineates the functional organization of the locomotor network.Brain structure & function · 2018Article
- Whole-organism cellular gene-expression atlas reveals conserved cell types in the ventral nerve cord ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes.Scientific reports · 2017Article
- Article
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Zebrafish intraspinal serotonergic neuron (ISN) morphology and distribution have been examined in detail at different ages; however, some aspects of the development of these cells remain unclear. Although antibodies to serotonin (5-HT) have detected ISNs in the ventral spinal cord of embryos, larvae, and adults, the only tryptophan hydroxylase (tph) transcript that has been described in the spinal cord is tph1a. Paradoxically, spinal tph1a is only expressed transiently in embryos, which brings the source of 5-HT in the ISNs of larvae and adults into question. Because the pet1 and tph2 promoters drive transgene expression in the spinal cord, we hypothesized that tph2 is expressed in spinal cords of zebrafish larvae. We confirmed this hypothesis through in situ hybridization. Next, we used 5-HT antibody labeling and transgenic markers of tph2-expressing neurons to identify a transient population of ISNs in embryos that was distinct from ISNs that appeared later in development. The existence of separate ISN populations may not have been recognized previously due to their shared location in the ventral spinal cord. Finally, we used transgenic markers and immunohistochemical labeling to identify the transient ISN population as GABAergic Kolmer-Agduhr double-prime (KA″) neurons. Altogether, this study revealed a novel developmental paradigm in which KA″ neurons are transiently serotonergic before the appearance of a stable population of tph2-expressing ISNs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.