ArticlePloS one2015
Mfa4, an Accessory Protein of Mfa1 Fimbriae, Modulates Fimbrial Biogenesis, Cell Auto-Aggregation, and Biofilm Formation in Porphyromonas gingivalis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Host insulin resistance caused byFrontiers in cellular and infection microbiology · 2023Pooled it
- Innovative strategies targeting oral microbial dysbiosis: unraveling mechanisms and advancing therapies for periodontitis.Frontiers in cellular and infection microbiology · 2025Review
- cAMP-independent Crp homolog adds to the multi-layer regulatory network inFrontiers in cellular and infection microbiology · 2025Article
- Local and Systemic Effects ofMicroorganisms · 2023Review
- Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae inJournal of oral microbiology · 2023Article
- HowMaterials (Basel, Switzerland) · 2022Review
- [Computer simulation of molecular docking between methylene blue and some proteins ofBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2022Article
- Porphyromonas gingivalis outside the oral cavity.Odontology · 2022Review
- Review
- Diversity analysis of genes encoding Mfa1 fimbrial components in Porphyromonas gingivalis strains.PloS one · 2021Article
- Article
- Identification of functional domains of the minor fimbrial antigen involved in the interaction of Porphyromonas gingivalis with oral streptococci.Molecular oral microbiology · 2020Article
- A Putative Type V Pilus Contributes to Bacteroides thetaiotaomicron Biofilm Formation Capacity.Journal of bacteriology · 2019Article
- In Vitro Effect of the Traditional Medicine Hainosan (Painongsan) onMedicines (Basel, Switzerland) · 2019Article
- Pathogenesis of Important Virulence Factors ofFrontiers in cellular and infection microbiology · 2019Review
- Effect ofBioMed research international · 2019Article
- Transcriptome analysis of Porphyromonas gingivalis and Acinetobacter baumannii in polymicrobial communities.Molecular oral microbiology · 2018Article
- Structural and functional characterization of shaft, anchor, and tip proteins of the Mfa1 fimbria from the periodontal pathogen Porphyromonas gingivalis.Scientific reports · 2018Article
- Maturation of the Mfa1 Fimbriae in the Oral PathogenFrontiers in cellular and infection microbiology · 2018Article
- Novel fimbrilin PGN_1808 in Porphyromonas gingivalis.PloS one · 2017Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Porphyromonas gingivalis, a gram-negative obligate anaerobic bacterium, is considered to be a key pathogen in periodontal disease. The bacterium expresses Mfa1 fimbriae, which are composed of polymers of Mfa1. The minor accessory components Mfa3, Mfa4, and Mfa5 are incorporated into these fimbriae. In this study, we characterized Mfa4 using genetically modified strains. Deficiency in the mfa4 gene decreased, but did not eliminate, expression of Mfa1 fimbriae. However, Mfa3 and Mfa5 were not incorporated because of defects in posttranslational processing and leakage into the culture supernatant, respectively. Furthermore, the mfa4-deficient mutant had an increased tendency to auto-aggregate and form biofilms, reminiscent of a mutant completely lacking Mfa1. Notably, complementation of mfa4 restored expression of structurally intact and functional Mfa1 fimbriae. Taken together, these results indicate that the accessory proteins Mfa3, Mfa4, and Mfa5 are necessary for assembly of Mfa1 fimbriae and regulation of auto-aggregation and biofilm formation of P. gingivalis. In addition, we found that Mfa3 and Mfa4 are processed to maturity by the same RgpA/B protease that processes Mfa1 subunits prior to polymerization.
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