ArticlePloS one2015
Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- A perfusable decellularized plant-based air-liquid-interface-on-a-chip for investigating inorganic dust aerosol exposure.Scientific reports · 2025Article
- Models to evaluate the pulmonary toxicity of desert dust and what we have learned from them so far: a mini-review.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Novel Approach Methodologies in Modeling Complex Bioaerosol Exposure in Asthma and Allergic Rhinitis Under Climate Change.Expert reviews in molecular medicine · 2025Review
- Advances and challenges in organ-on-chip technology: toward mimicking human physiology and disease in vitro.Medical & biological engineering & computing · 2024Review
- Review
- An Insight on Microfluidic Organ-on-a-Chip Models for PMACS omega · 2024Review
- Droplet fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip system.RSC advances · 2023Article
- Easy-to-Build and Reusable Microfluidic Device for the Dynamic Culture of Human Bronchial Cystic Fibrosis Epithelia.ACS biomaterials science & engineering · 2023Article
- Advanced Microfluidic Vascularized Tissues as Platform for the Study of Human Diseases and Drug Development.Current neuropharmacology · 2023Article
- Human milk extracellular vesicles preserve bronchial epithelial barrier integrity and reduce TLR3-induced inflammationJournal of extracellular biology · 2022Article
- Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system.Biomicrofluidics · 2022Article
- Article
- Organ-on-chip applications in drug discovery: an end user perspective.Biochemical Society transactions · 2021Review
- PerfuPul-A Versatile Perfusable Platform to Assess Permeability and Barrier Function of Air Exposed Pulmonary Epithelia.Frontiers in bioengineering and biotechnology · 2021Article
- Organ-on-a-chip: recent breakthroughs and future prospects.Biomedical engineering online · 2020Review
- Advanced in vitro lung-on-chip platforms for inhalation assays: From prospect to pipeline.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · 2019Review
- Capturing the Onset of Bacterial Pulmonary Infection in Acini-On-Chips.Advanced biosystems · 2019Article
- New Age Strategies To Reconstruct Mucosal Tissue Colonization and Growth in Cell Culture Systems.Microbiology spectrum · 2019Review
- Biomimetics of the pulmonary environmentBiomicrofluidics · 2018Article
- Through the Looking Glass:Applied in vitro toxicology · 2018Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The airway epithelium is exposed to a variety of harmful agents during breathing and appropriate cellular responses are essential to maintain tissue homeostasis. Recent evidence has highlighted the contribution of epithelial barrier dysfunction in the development of many chronic respiratory diseases. Despite intense research efforts, the responses of the airway barrier to environmental agents are not fully understood, mainly due to lack of suitable in vitro models that recapitulate the complex in vivo situation accurately. Using an interdisciplinary approach, we describe a novel dynamic 3D in vitro model of the airway epithelium, incorporating fully differentiated primary human airway epithelial cells at the air-liquid interface and a basolateral microfluidic supply of nutrients simulating the interstitial flow observed in vivo. Through combination of the microfluidic culture system with an automated fraction collector the kinetics of cellular responses by the airway epithelium to environmental agents can be analysed at the early phases for the first time and with much higher sensitivity compared to common static in vitro models. Following exposure of primary differentiated epithelial cells to pollen we show that CXCL8/IL-8 release is detectable within the first 2h and peaks at 4-6h under microfluidic conditions, a response which was not observed in conventional static culture conditions. Such a microfluidic culture model is likely to have utility for high resolution temporal profiling of toxicological and pharmacological responses of the airway epithelial barrier, as well as for studies of disease mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.