Evidence map›Paper›PMID 26435337›Full record

ArticleVirus research2016

Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf.

Elisabete Takiuchi, Rubia Macedo, Andressa Fernanda Kunz, Jessica Cristhine Gallego, Janaina Lustosa de Mello, Rodrigo Alejandro Arellano Otonel, Amauri Alcindo Alfieri

Open access · greenAbstract read
In one paragraph

Article in Virus research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Elisabete TakiuchiDepartment of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil. Electronic address: e.takiuchi@ufpr.br.
Rubia MacedoDepartment of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil.
Andressa Fernanda KunzDepartment of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil.
Jessica Cristhine GallegoDepartment of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil.
Janaina Lustosa de MelloDepartment of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil.
Rodrigo Alejandro Arellano OtonelDepartment of Preventive Veterinary Medicine, State University of Londrina - UEL, PO Box 6001, 86051-990, Londrina, PR, Brazil.
Amauri Alcindo AlfieriDepartment of Preventive Veterinary Medicine, State University of Londrina - UEL, PO Box 6001, 86051-990, Londrina, PR, Brazil.
Universidade Federal do Paraná · BRUniversidade Estadual de Londrina · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Picobirnavirus (PBV) belongs to the family Picobirnaviridae. PBV are a group of emerging non-enveloped viruses, with a bisegmented double-stranded RNA genome that can infect a wide range of hosts. This study reports the occurrence of PBV in fecal samples from five Brazilian dairy cattle herds. From the 289 stool samples of individual calves analyzed by silver-stained polyacrylamide gel electrophoresis (ss-PAGE) the PBV was detected in 8.3 % (24/289), of which 10.2% (18/176) had diarrheic consistency. Of the 24 positive samples in ss-PAGE, 5 (20.8%) of them showed a small electrophoretic profile and 19 (79.2%) samples had large profile. From the 24 positives samples by ss-PAGE, 15 (62.5%) were successfully amplified (201 bp) using GI specific primers targeting the RdRp gene of PBV. The analysis of nucleotide identity matrix revealed that the bovine PBV strain identified in this study, showed the highest nucleotide identity (81%) with PBV strain detected in turkey (MD-2010/HM803965). This is the first nucleotide sequence of a bovine PBV strain in the American continent and the first detection of small genome profile of PBV-like strains in bovine hosts.

Indexed as

AnimalsBrazilCattleCattle DiseasesDiarrheaElectrophoresis, Polyacrylamide GelFecesPhylogenyPicobirnavirusRNA, ViralRNA Virus InfectionsRNA, ViralBovinePAGEPhylogenyPicobirnavirusRT-PCR

Identifiers

PMID26435337
PMCPMC7127629
OpenAlexW1876993103

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.