Evidence map›Paper›PMID 26435141›Full record

ArticleFEBS letters2015

Asparagine deamidation reduces DNA-binding affinity of the Drosophila melanogaster Scr homeodomain.

Nichole E O'Connell, Katherine Lelli, Richard S Mann, Arthur G Palmer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Nichole E O'ConnellDepartment of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States.
Katherine LelliDepartment of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States.
Richard S MannDepartment of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States.
Arthur G PalmerDepartment of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States. Electronic address: agp6@columbia.edu.
Columbia University · US

Funding

PREDOCTORAL TRAINING GRANT IN HUMAN GENETICS &DEVELOPMET32GM007088 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SHEN, MICHAEL M. · 1985 to 2020
$7.1M
Interpreting and Deploying Genomic Information During Animal DevelopmentR35GM118336 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI RICHARD S MANN · 2016 to 2026
$6.9M
STRUCTURE AND DYNAMICS OF RIBONUCLEASE HR01GM050291 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI PALMER, ARTHUR G · 1994 to 2017
$5.6M
MOLECULAR GENETICS OF SEGMENT IDENTITYR01GM054510 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MANN, RICHARD S · 1996 to 2015
$5.5M
TRAINING PROGRAM IN MOLECULAR BIOPHYSICST32GM008281 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI PALMER, ARTHUR G · 1988 to 2017
$5.4M
NIGMS NIH HHS R01 GM050291NIGMS NIH HHS R01GM050291NIGMS NIH HHS R01 GM054510NIGMS NIH HHS R01GM054510NIGMS NIH HHS R35 GM118336NIGMS NIH HHS T32 GM007088NIGMS NIH HHS T32 GM008281NIGMS NIH HHS T32GM008281
6 · The paper itself

Abstract

Spontaneous deamidation of asparagine is a non-enzymatic post-translational modification of proteins. Residue Asn 321 is the main site of deamidation of the Drosophila melanogaster Hox transcription factor Sex Combs Reduced (Scr). Formation of iso-aspartate, the major deamidation product, is detected by HNCACB triple-resonance NMR spectroscopy. The rate of deamidation is quantified by fitting the decay of Asn NH2 side-chain signals in a time-series of (15)N-(1)H HSQC NMR spectra. The deamidated form of Scr binds to specific DNA target sequences with reduced affinity as determined by an electrophoretic mobility shift assay.

Indexed as

AnimalsAsparagineBinding SitesDNADrosophila melanogasterDrosophila ProteinsIsoaspartic AcidMagnetic Resonance SpectroscopyProtein BindingProtein Processing, Post-TranslationalTranscription FactorsAsparagineDNADrosophila ProteinsIsoaspartic AcidScr protein, DrosophilaTranscription FactorsAsparagine deamidationDissociation constantDNA bindingHox transcription factorNMR spectroscopySex Combs Reduced

Identifiers

PMID26435141
PMCPMC4674082
OpenAlexW1857688987

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.