ArticleMicrobiologyOpen2015
Characterization of Mycobacterium smegmatis sigF mutant and its regulon: overexpression of SigF antagonist (MSMEG_1803) in M. smegmatis mimics sigF mutant phenotype, loss of pigmentation, and sensitivity to oxidative stress.
Article in MicrobiologyOpen, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Advances and Challenges in Mycobacterial Genetic Engineering: Techniques for Knockout, Knockdown and Overexpression.Journal of microbiology and biotechnology · 2025Review
- MoaB2, a newly identified transcription factor, binds to σJournal of bacteriology · 2024Article
- MnoSR removal inFrontiers in cellular and infection microbiology · 2024Article
- Dynamic Transcriptional Landscape ofInternational journal of molecular sciences · 2023Article
- The C-Terminal Acid Phosphatase Module of the RNase HI Enzyme RnhC Controls Rifampin Sensitivity and Light-Dependent Colony Pigmentation of Mycobacterium smegmatis.Journal of bacteriology · 2023Article
- Mycobacterial Regulatory Systems Involved in the Regulation of Gene Expression Under Respiration-Inhibitory Conditions.Journal of microbiology (Seoul, Korea) · 2023Review
- Insights into the evolutionary history of the virulent factor HBHA of Mycobacterium tuberculosis.Archives of microbiology · 2021Article
- STAS Domain Only Proteins in Bacterial Gene Regulation.Frontiers in cellular and infection microbiology · 2021Review
- Single START-domain protein Mtsp17 is involved in transcriptional regulation in Mycobacterium smegmatis.PloS one · 2021Article
- Effect of the TetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona.Microbial cell factories · 2020Article
- Post-transcriptional modulation of the SigF regulon in Mycobacterium smegmatis by the PhoH2 toxin-antitoxin.PloS one · 2020Article
- Induction of theFrontiers in microbiology · 2020Article
- Brought to you courtesy of the red, white, and blue-pigments of nontuberculous mycobacteria.AIMS microbiology · 2020Article
- The Partner Switching System of the SigF Sigma Factor inFrontiers in microbiology · 2020Article
- Tripartite Regulation of theJournal of bacteriology · 2019Article
- Mutational loss of carotenoids in alkaliphilicMicrobiology (Reading, England) · 2019Article
- Defining the Transcriptional and Post-transcriptional Landscapes ofFrontiers in microbiology · 2019Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Mycobacterium smegmatis, sigF is widely expressed during different growth stages and plays role in adaptation to stationary phase and oxidative stress. Using a sigF deletion mutant of M. smegmatis mc(2) 155, we demonstrate that SigF is not essential for growth of bacterium. Deletion of sigF results in loss of carotenoid pigmentation which rendered increased susceptibility to H2 O2 induced oxidative stress in M. smegmatis. SigF modulates the cell surface architecture and lipid biosynthesis extending the repertoire of SigF function in this species. M. smegmatis SigF regulon included variety of genes expressed during exponential and stationary phases of growth and those responsible for oxidative stress, lipid biosynthesis, energy, and central intermediary metabolism. Furthermore, we report the identification of a SigF antagonist, an anti-sigma factor (RsbW), which upon overexpression in M. smegmatis wild type strain produced a phenotype similar to M. smegmatis mc(2) 155 ΔsigF strain. The SigF-anti-SigF interaction is duly validated using bacterial two-hybrid and pull down assays. In addition, anti-sigma factor antagonists, RsfA and RsfB were identified and their interactions with anti-sigma factor were experimentally validated. Identification of these proteins will help decode regulatory circuit of this alternate sigma factor.
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