ArticleJournal of neuroscience methods2016
Use of von Frey filaments to assess nociceptive sensitization in the hornworm, Manduca sexta.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Caterpillars suppress nocifensive behaviours during the quiescent 'sphinx' state.Biology letters · 2025Article
- Robust behavioral assessment of the inducible Friedreich's ataxia mouse does not show improvement with NRF2 induction.Disease models & mechanisms · 2025Article
- Injury alters sensory, motor, and integrative elements underlying operant conditioning in the medicinal leech.PloS one · 2025Article
- Characterization of a rapid avoidance behavior in Manduca sexta larvae in response to noxious stimuli.The Journal of experimental biology · 2024Article
- Muscle in the caterpillar Manduca sexta responds to an immune challenge, but at a cost, suggesting a physiological trade-off.The Journal of experimental biology · 2023Article
- Serotonin mediates stress-like effects on responses to non-nociceptive stimuli in the medicinal leech Hirudo verbana.The Journal of experimental biology · 2022Article
- Approaches to studying injury-induced sensitization and the potential role of an endocannabinoid transmitter.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2022Article
- A Novel Cellular Therapy to Treat Pancreatic Pain in Experimental Chronic Pancreatitis Using Human Alpha-1 Antitrypsin Overexpressing Mesenchymal Stromal Cells.Biomedicines · 2021Article
- Mechanical Nociceptive Threshold, Tissue Alterations and Horn Growth in Calves after Injection of Isoeugenol or Clove Oil under the Horn Bud.Animals : an open access journal from MDPI · 2021Article
- Compression of The Sciatic Nerve May not Contribute to Ipsilateral Hyperalgesia Development in Ovariectomized Female Rats!Cell journal · 2021Article
- Nociceptive Threshold of Calves and Goat Kids Undergoing Injection of Clove Oil or Isoeugenol for Disbudding.Animals : an open access journal from MDPI · 2020Article
- Animals have a Plan B: how insects deal with the dual challenge of predators and pathogens.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2020Review
- Listening to your gut: immune challenge to the gut sensitizes body wall nociception in the caterpillarPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Nociceptive Biology of Molluscs and Arthropods: Evolutionary Clues About Functions and Mechanisms Potentially Related to Pain.Frontiers in physiology · 2018Review
- Central neural alterations predominate in an insect model of nociceptive sensitization.The Journal of comparative neurology · 2017Article
- Comparative biology of pain: What invertebrates can tell us about how nociception works.Journal of neurophysiology · 2017Review
- Neurobehavioral deficits in the KIKO mouse model of Friedreich's ataxia.Behavioural brain research · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe hornworm Manduca sexta exhibits a defensive strike to noxious assaults, a response that is robust and is easily observed by experimenters. Von Frey filaments and methods typical for studying nociception in other animals were used to assess the strike response in M. sexta. NEW
methodsA series of von Frey filaments was applied to the body wall in ascending order and the data generated were used to determine the strike threshold by (i) the up-and-down method, (ii) the first response method, and (iii) the simplified up-and-down order method (SUDO). The effect of a noxious pinch on strike threshold was assessed. COMPARISON WITH EXISTING
methodsTo our knowledge none of these methods has been used on M. sexta previously, making the use of the up-and-down and SUDO methods the first in an invertebrate. The use of the first response method has been used in other invertebrates, and the method appears equally suited to M. sexta.
resultsAll three methods were successful in monitoring the threshold sensitivity to touch, which was lowered (sensitized) by tissue damage induced with a pinch. Sensitization lasted 19h.
conclusionsAll three methods of assessing nociception were successfully applied to quantify the defensive strike response in M. sexta, although the SUDO method required empirical assessment of which filament to start the test sequence with. The results revealed both short- and long-term sensitization. These methods should prove to be useful for quantifying sensitization in M. sexta.
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