ArticleThe Plant cell2015
Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
- FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques.Plant methods · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- VISUAL-PH and VISUAL-CC: Modified Culture Systems for Phloem Differentiation in Arabidopsis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- EXO70A1 governs both the timing and patterning of secondary cell wall deposition: evidence from anFrontiers in plant science · 2026Article
- A needle in the haystack: Single-cell omics of the distinct xylem differentiation programs in gymnosperms and angiosperms.The Plant cell · 2025Article
- Physiological roles of lignins - tuning cell wall hygroscopy and biomechanics.The New phytologist · 2025Review
- MicroRNA analysis reveals 2 modules that antagonistically regulate xylem tracheary element development in Arabidopsis.The Plant cell · 2024Article
- Heterogeneity in Mechanical Properties of Plant Cell Walls.Plants (Basel, Switzerland) · 2024Review
- Single-cell transcriptomics: a new frontier in plant biotechnology research.Plant cell reports · 2024Review
- Microtubule-associated phase separation of MIDD1 tunes cell wall spacing in xylem vessels in Arabidopsis thaliana.Nature plants · 2024Article
- Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis.The Plant cell · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Plant biomechanics and resilience to environmental changes are controlled by specific lignin chemistries in each vascular cell type and morphotype.The Plant cell · 2022Article
- Current Understanding of the Genetics and Molecular Mechanisms Regulating Wood Formation in Plants.Genes · 2022Review
- Secondary cell wall patterning-connecting the dots, pits and helices.Open biology · 2022Article
- A rich and bountiful harvest: Key discoveries in plant cell biology.The Plant cell · 2022Review
- Tracheary elements from calli of Japanese horse chestnut (Aesculus turbinata) form perforation-like structures.Planta · 2021Article
- HSP70-3 Interacts with Phospholipase Dδ and Participates in Heat Stress Defense.Plant physiology · 2021Article
- Long-term single-cell imaging and simulations of microtubules reveal principles behind wall patterning during proto-xylem development.Nature communications · 2021Article
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7 authors at 2 institutions in 2 countries.
Funding
Abstract
Plant vascular cells, or tracheary elements (TEs), rely on circumferential secondary cell wall thickenings to maintain sap flow. The patterns in which TE thickenings are organized vary according to the underlying microtubule bundles that guide wall deposition. To identify microtubule interacting proteins present at defined stages of TE differentiation, we exploited the synchronous differentiation of TEs in Arabidopsis thaliana suspension cultures. Quantitative proteomic analysis of microtubule pull-downs, using ratiometric (14)N/(15)N labeling, revealed 605 proteins exhibiting differential accumulation during TE differentiation. Microtubule interacting proteins associated with membrane trafficking, protein synthesis, DNA/RNA binding, and signal transduction peaked during secondary cell wall formation, while proteins associated with stress peaked when approaching TE cell death. In particular, CELLULOSE SYNTHASE-INTERACTING PROTEIN1, already associated with primary wall synthesis, was enriched during secondary cell wall formation. RNAi knockdown of genes encoding several of the identified proteins showed that secondary wall formation depends on the coordinated presence of microtubule interacting proteins with nonoverlapping functions: cell wall thickness, cell wall homogeneity, and the pattern and cortical location of the wall are dependent on different proteins. Altogether, proteins linking microtubules to a range of metabolic compartments vary specifically during TE differentiation and regulate different aspects of wall patterning.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.