ArticleNucleic acids research2015
Assembly and analysis of eukaryotic Argonaute-RNA complexes in microRNA-target recognition.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- A spontaneous thermo-sensitive female sterility mutation in rice enables fully mechanized hybrid breeding.Cell research · 2022Article
- Different N-Glycosylation Sites Reduce the Activity of Recombinant DSPAα2.Current issues in molecular biology · 2022Article
- 3D Modeling of Non-coding RNA Interactions.Advances in experimental medicine and biology · 2022Article
- Structural Modeling of the SARS-CoV-2 Spike/Human ACE2 Complex Interface can Identify High-Affinity Variants Associated with Increased Transmissibility.Journal of molecular biology · 2021Article
- Germline AGO2 mutations impair RNA interference and human neurological development.Nature communications · 2020Article
- miR-122 and Ago interactions with the HCV genome alter the structure of the viral 5' terminus.Nucleic acids research · 2019Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- A non-canonical site reveals the cooperative mechanisms of microRNA-mediated silencing.Nucleic acids research · 2017Article
- TargetLink, a new method for identifying the endogenous target set of a specific microRNA in intact living cells.RNA biology · 2017Article
- MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Experimental studies have uncovered a variety of microRNA (miRNA)-target duplex structures that include perfect, imperfect and seedless duplexes. However, non-canonical binding modes from imperfect/seedless duplexes are not well predicted by computational approaches, which rely primarily on sequence and secondary structural features, nor have their tertiary structures been characterized because solved structures to date are limited to near perfect, straight duplexes in Argonautes (Agos). Here, we use structural modeling to examine the role of Ago dynamics in assembling viable eukaryotic miRNA-induced silencing complexes (miRISCs). We show that combinations of low-frequency, global modes of motion of Ago domains are required to accommodate RNA duplexes in model human and C. elegans Ago structures. Models of viable miRISCs imply that Ago adopts variable conformations at distinct target sites that generate distorted, imperfect miRNA-target duplexes. Ago's ability to accommodate a duplex is dependent on the region where structural distortions occur: distortions in solvent-exposed seed and 3'-end regions are less likely to produce steric clashes than those in the central duplex region. Energetic analyses of assembled miRISCs indicate that target recognition is also driven by favorable Ago-duplex interactions. Such structural insights into Ago loading and target recognition mechanisms may provide a more accurate assessment of miRNA function.
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Registered trials
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