ArticleMicrobes and infection2016
Development of dual fluorescent stage specific reporter strain of Toxoplasma gondii to follow tachyzoite and bradyzoite development in vitro and in vivo.
Article in Microbes and infection, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Kinase inhibitors in organoid media influenceMicrobiology spectrum · 2026Article
- Comprehensive diagnostic approaches to feline toxoplasmosis: Bridging traditional methods and emerging technologies.Virulence · 2025Review
- Modeling Toxoplasma gondii-gut early interactions using a human microphysiological system.PLoS neglected tropical diseases · 2025Article
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- Host cell-specific metabolism of linoleic acid controlsInfection and immunity · 2024Article
- Host cell-specific metabolism of linoleic acid controlsbioRxiv : the preprint server for biology · 2024Article
- Iron Stress Affects the Growth and Differentiation ofInternational journal of molecular sciences · 2024Article
- FromiScience · 2024Article
- Inflammatory response and parasite regulation in acute toxoplasmosis: the role of P2X7 receptor in controlling virulent atypical genotype strain ofFrontiers in immunology · 2024Article
- Animal Models for Toxoplasma gondii Infection.Current protocols · 2023Article
- The determinants regulatingFrontiers in microbiology · 2022Review
- Key Limitations and New Insights Into theFrontiers in cellular and infection microbiology · 2020Review
- Development of an in vitro system to study the developmental stages of Toxoplasma gondii using a genetically modified strain expressing markers for tachyzoites and bradyzoites.Parasitology research · 2019Article
- Drugs in development for toxoplasmosis: advances, challenges, and current status.Drug design, development and therapy · 2017Review
- The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth.Parasite (Paris, France) · 2017Article
- Imaging flow cytometry analysis of intracellular pathogens.Methods (San Diego, Calif.) · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Toxoplasma gondii is a protozoan that infects 30% of humans as intermediate hosts. T Sexual reproduction can occur only within the intestinal tract of felines, however, infection in other mammals and birds is associated with asexual replication and interconversion between the tachyzoite and bradyzoite stages. Bradyzoites are slow growing forms found in tissue cysts in latent infection. Recently, our group described the biological behavior of the EGS strain that forms thick walled cysts spontaneously in tissue culture, constituting a useful tool for examining the developmental biology of T. gondii. To further improve the usefulness of this model, we constructed genetically modified EGS parasites that express fluorescent tags under the control of stage specific promoters. The promoter regions for SAG-1 (tachyzoite specific), BAG-1 and LDH-2 (bradyzoite specific) were amplified by PCR and plasmids were constructed with mCherry (redT) and sfGFP (greenB) sequences, respectively. Strains of parasites were selected using FACS to arrive at single fluorescent and dual fluorescent strains of EGS expressing tags in a stage specific manner. In cell cultures, vacuoles labeled by immunofluorescence assay using anti-CST-1 a marker for T. gondii cyst wall contained parasites that were positive for BAG1-GFP and negative for SAG1-mCherry. Tachyzoites and bradyzoites harvested from the mice expressed stage specific mCherry and GFP proteins, respectively. These new dual fluorescent transgenic EGS strains are a promising tool to elucidate the mechanisms of T. gondii differentiation both in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.