ArticlePloS one2015
Host-Associated Metagenomics: A Guide to Generating Infectious RNA Viromes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Editorial Note: Host-Associated Metagenomics: A Guide to Generating Infectious RNA Viromes.PloS one · 2026Article
- The Role of Hematophagous Arthropods, Other than Mosquitoes and Ticks, in Arbovirus Transmission.Viruses · 2025Review
- Alterations of the gut microbiome in HIV infection highlight human anelloviruses as potential predictors of immune recovery.Microbiome · 2024Article
- Application of Host-Depleted Nanopore Metagenomic Sequencing in the Clinical Detection of Pathogens in Pigs and Cats.Animals : an open access journal from MDPI · 2023Article
- Expression of Concern: Host-Associated Metagenomics: A Guide to Generating Infectious RNA Viromes.PloS one · 2022Article
- Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021Review
- Analysis of Different Size Fractions Provides a More Complete Perspective of Viral Diversity in a Freshwater Embayment.Applied and environmental microbiology · 2021Article
- Virome Diversity among Mosquito Populations in a Sub-Urban Region of Marseille, France.Viruses · 2021Article
- Metagenomic sequencing of stool samples in Bangladeshi infants: virome association with poliovirus shedding after oral poliovirus vaccination.Scientific reports · 2020Article
- Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance.Nature microbiology · 2020Article
- New Isolates of Pandoraviruses: Contribution to the Study of Replication Cycle Steps.Journal of virology · 2019Article
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- Article
- Laboratory Methods in Molecular Epidemiology: Viral Infections.Microbiology spectrum · 2018Review
- Metagenomics detection and characterisation of viruses in faecal samples from Australian wild birds.Scientific reports · 2018Article
- A decade of RNA virus metagenomics is (not) enough.Virus research · 2018Review
- Screening for Viral Pathogens in African Simian Bushmeat Seized at A French Airport.Transboundary and emerging diseases · 2017Article
- Efficient and unbiased metagenomic recovery of RNA virus genomes from human plasma samples.Scientific reports · 2017Article
- Viral Metagenomics on Blood-Feeding Arthropods as a Tool for Human Disease Surveillance.International journal of molecular sciences · 2016Review
- Characterization of Viral Communities of Biting Midges and Identification of Novel Thogotovirus Species and Rhabdovirus Genus.Viruses · 2016Article
Corrections and comments
- Expression of concern · 2022-12-13Investigation by Journal/Publisher ·
- Commented on by
- Expression of concern
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetagenomic analyses have been widely used in the last decade to describe viral communities in various environments or to identify the etiology of human, animal, and plant pathologies. Here, we present a simple and standardized protocol that allows for the purification and sequencing of RNA viromes from complex biological samples with an important reduction of host DNA and RNA contaminants, while preserving the infectivity of viral particles. PRINCIPAL
findingsWe evaluated different viral purification steps, random reverse transcriptions and sequence-independent amplifications of a pool of representative RNA viruses. Viruses remained infectious after the purification process. We then validated the protocol by sequencing the RNA virome of human body lice engorged in vitro with artificially contaminated human blood. The full genomes of the most abundant viruses absorbed by the lice during the blood meal were successfully sequenced. Interestingly, random amplifications differed in the genome coverage of segmented RNA viruses. Moreover, the majority of reads were taxonomically identified, and only 7-15% of all reads were classified as "unknown", depending on the random amplification method.
conclusionThe protocol reported here could easily be applied to generate RNA viral metagenomes from complex biological samples of different origins. Our protocol allows further virological characterizations of the described viral communities because it preserves the infectivity of viral particles and allows for the isolation of viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.