ArticleThe Journal of biological chemistry2015
Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 81 citations in OpenAlex.
- Cell cycle-dependent chromatin motion: a role for DNA content doubling over cohesion.Nucleic acids research · 2026Article
- Telomere shortening in laminopathic dilated cardiomyopathy.NPJ Regenerative medicine · 2026Article
- Nuclear cholesterol regulates nuclear size and DNA damage responses in cancer stem cells.Neuro-oncology · 2025Article
- How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025Review
- Tubular Lamin- and Emerin-Lined Nuclear Envelope Invaginations Isolate Chromatin Domains for Gene Expression Regulation.Sub-cellular biochemistry · 2025Review
- Review
- Protein kinase C activity modulates nuclear Lamin A/C dynamics in HeLa cells.Scientific reports · 2024Article
- A high-content screen reveals new regulators of nuclear membrane stability.Scientific reports · 2024Article
- Nuclear growth and import can be uncoupled.Molecular biology of the cell · 2024Article
- A high-content screen reveals new regulators of nuclear membrane stability.bioRxiv : the preprint server for biology · 2023Article
- Article
- Age-dependent Lamin changes induce cardiac dysfunction via dysregulation of cardiac transcriptional programs.Nature aging · 2023Article
- Differential contribution of nuclear size scaling mechanisms between Xenopus species.Development, growth & differentiation · 2022Article
- The Nuclear-to-Cytoplasmic Ratio: Coupling DNA Content to Cell Size, Cell Cycle, and Biosynthetic Capacity.Annual review of genetics · 2022Review
- Nuclear F-actin and Lamin A antagonistically modulate nuclear shape.Journal of cell science · 2022Article
- Chemical Interrogation of Nuclear Size Identifies Compounds with Cancer Cell Line-Specific Effects on Migration and Invasion.ACS chemical biology · 2022Article
- An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.The Journal of experimental biology · 2022Article
- Specificity of Nuclear Size Scaling in Frog Erythrocytes.Frontiers in cell and developmental biology · 2022Article
- Nuclear size rectification: A potential new therapeutic approach to reduce metastasis in cancer.Frontiers in cell and developmental biology · 2022Review
- Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer.Oncology letters · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A fundamental question in cell biology concerns the regulation of organelle size. While nuclear size is exquisitely controlled in different cell types, inappropriate nuclear enlargement is used to diagnose and stage cancer. Clarifying the functional significance of nuclear size necessitates an understanding of the mechanisms and proteins that control nuclear size. One structural component implicated in the regulation of nuclear morphology is the nuclear lamina, a meshwork of intermediate lamin filaments that lines the inner nuclear membrane. However, there has not been a systematic investigation of how the level and type of lamin expression influences nuclear size, in part due to difficulties in precisely controlling lamin expression levels in vivo. In this study, we circumvent this limitation by studying nuclei in Xenopus laevis egg and embryo extracts, open biochemical systems that allow for precise manipulation of lamin levels by the addition of recombinant proteins. We find that nuclear growth and size are sensitive to the levels of nuclear lamins, with low and high concentrations increasing and decreasing nuclear size, respectively. Interestingly, each type of lamin that we tested (lamins B1, B2, B3, and A) similarly affected nuclear size whether added alone or in combination, suggesting that total lamin concentration, and not lamin type, is more critical to determining nuclear size. Furthermore, we show that altering lamin levels in vivo, both in Xenopus embryos and mammalian tissue culture cells, also impacts nuclear size. These results have implications for normal development and carcinogenesis where both nuclear size and lamin expression levels change.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.