ArticleThe Journal of cell biology1989
The organization of myosin and actin in rapid frozen nerve growth cones.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 212 citations in OpenAlex.
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- Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction.Communications biology · 2020Article
- Neurexin-1α regulates neurite growth of rat hippocampal neurons.International journal of physiology, pathophysiology and pharmacology · 2019Article
- Differential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones.Neural development · 2015Article
- Ionizing radiation effects on the secretory-stage ameloblasts and enamel organic extracellular matrix.Radiation and environmental biophysics · 2014Article
- Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones.Molecular biology of the cell · 2013Article
- Optogenetic control of PIP3: PIP3 is sufficient to induce the actin-based active part of growth cones and is regulated via endocytosis.PloS one · 2013Article
- Single vesicle imaging indicates distinct modes of rapid membrane retrieval during nerve growth.BMC biology · 2012Article
- The role of actin turnover in retrograde actin network flow in neuronal growth cones.PloS one · 2012Article
- Gelsolin in cerebrospinal fluid as a potential biomarker of epilepsy.Neurochemical research · 2011Article
- Molecular motor proteins and amyotrophic lateral sclerosis.International journal of molecular sciences · 2011Review
- Phosphorylation of ezrin/radixin/moesin proteins by LRRK2 promotes the rearrangement of actin cytoskeleton in neuronal morphogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Topography and nanomechanics of live neuronal growth cones analyzed by atomic force microscopy.Biophysical journal · 2009Article
- Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.Molecular biology of the cell · 2008Article
- Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions by "in vivo cryotechnique".Histochemistry and cell biology · 2006Article
- Guiding neuronal growth cones using Ca2+ signals.Trends in cell biology · 2004Review
- Myosin IIA drives neurite retraction.Molecular biology of the cell · 2003Article
- Actin turnover is required to prevent axon retraction driven by endogenous actomyosin contractility.The Journal of cell biology · 2002Article
- Focal loss of actin bundles causes microtubule redistribution and growth cone turning.The Journal of cell biology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rapid freezing and freeze substitution were used in conjunction with immunofluorescence, whole mount EM, and immunoelectron microscopy to study the organization of myosin and actin in growth cones of cultured rat superior cervical ganglion neurons. The general cytoplasmic organization was determined by whole mount EM; tight microfilament bundles formed the core of filopodia while a dense meshwork formed the underlying structure of lamellipodia. Although the central microtubule and organelle-rich region of the growth cone had fewer microfilaments, dense foci and bundles of microfilaments were usually observed. Anti-actin immunofluorescence and rhodamine phalloidin staining of f-actin both showed intense staining of filopodia and lamellipodia. In addition, staining of bundles and foci were observed in central regions suggesting that the majority of the microfilaments seen by whole mount EM are actin filaments. Anti-myosin immunofluorescence was brightest in the central region and usually had a punctate pattern. Although less intense, anti-myosin staining was also seen in peripheral regions; it was most prominent at the border with the central region, in portions of lamellipodia undergoing ruffling, and in spots along the shaft and at the base of filopodia. Immunoelectron microscopy of myosin using postembedment labeling with colloidal gold showed a similar distribution to that seen by immunofluorescence. Label was scattered throughout the growth cone, but present as distinct aggregates in the peripheral region mainly along the border with the central region. Less frequently, aggregates were also seen centrally and along the shaft and at the base of filopodia. This distribution is consistent with myosins involvement in the production of tension and movements of growth cone filopodia and lamellipodia that occur during active neurite elongation.
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