ArticleThe Journal of cell biology1989
Nocodazole, a microtubule-active drug, interferes with apical protein delivery in cultured intestinal epithelial cells (Caco-2).
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 178 citations in OpenAlex.
- Endosomes and Microtubles are Required for Productive Infection in Aquareovirus.Virologica Sinica · 2020Article
- Cyclin-dependent kinase 5 regulates proliferation, migration, tyrosinase activity, and melanin production in B16-F10 melanoma cells via the essential regulator p-CREB.In vitro cellular & developmental biology. Animal · 2019Article
- Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Molecular biology of the cell · 2018Article
- Mechanisms of DRA recycling in intestinal epithelial cells: effect of enteropathogenic E. coli.American journal of physiology. Cell physiology · 2015Article
- Turnover of the actomyosin complex in zebrafish embryos directs geometric remodelling and the recruitment of lipid droplets.Scientific reports · 2015Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC.The Journal of cell biology · 2010Article
- PH-domain-dependent selective transport of p75 by kinesin-3 family motors in non-polarized MDCK cells.Journal of cell science · 2010Article
- Lovastatin inhibits the thrombin-induced loss of barrier integrity in bovine corneal endothelium.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2010Article
- Microtubule disassembly breaks down the barrier integrity of corneal endothelium.Experimental eye research · 2009Article
- Change in localization of alkaline phosphatase and mannosidase II by colchicine treatment of primary cultures of fetal rat hepatocytes.Acta histochemica et cytochemica · 2008Article
- Polarization-dependent selective transport to the apical membrane by KIF5B in MDCK cells.Developmental cell · 2007Article
- Dystroglycan receptor is involved in integrin activation in intestinal epithelia.American journal of physiology. Gastrointestinal and liver physiology · 2006Article
- Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells.Molecular biology of the cell · 1998Article
- GalNAc-alpha-O-benzyl inhibits NeuAcalpha2-3 glycosylation and blocks the intracellular transport of apical glycoproteins and mucus in differentiated HT-29 cells.The Journal of cell biology · 1998Article
- Apiconuclear organization of microtubules does not specify protein delivery from the trans-Golgi network to different membrane domains in polarized epithelial cells.Molecular biology of the cell · 1998Article
- Substrate recognition by osteoclast precursors induces C-src/microtubule association.The Journal of cell biology · 1997Article
- Bacteroides fragilis toxin rapidly intoxicates human intestinal epithelial cells (HT29/C1) in vitro.Infection and immunity · 1996Article
- Involvement of the mutated M protein in altered budding polarity of a pantropic mutant, F1-R, of Sendai virus.Journal of virology · 1996Article
- Polarized distribution of actin isoforms in gastric parietal cells.Molecular biology of the cell · 1995Article
Corrections and comments
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polarized delivery of membrane proteins to the cell surface and the initial secretion of lysosomal proteins into the culture medium were studied in the polarized human intestinal adenocarcinoma cell line Caco-2 in the presence or absence of the microtubule-active drug nocodazole. The appearance of newly synthesized proteins at the plasma membrane was measured by their sensitivity to proteases added either to the apical or the basolateral surface of cells grown on nitrocellulose filters. Nocodazole was found to reduce the delivery to the cell surface of an apical membrane protein, aminopeptidase N, and to lead to its partial missorting to the basolateral surface, whereas the drug had no influence on the delivery of a basolateral 120-kD membrane protein defined by a monoclonal antibody. Furthermore, nocodazole selectively blocked the apical secretion of two lysosomal proteins, cathepsin D and acid alpha-glucosidase, whereas the drug had no influence on their basolateral secretion. These results suggest that in Caco-2 cells an intact microtubular network is important for the transport of newly synthesized proteins to the apical cell surface.
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