ArticleEuropean journal of histochemistry : EJH2015
Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.
Article in European journal of histochemistry : EJH, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 71 citations in OpenAlex.
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- Mitochondrial malfunction and atrophy of astrocytes in the aged human cerebral cortex.Nature communications · 2023Article
- Commonly Overlooked Factors in Biocompatibility Studies of Neural Implants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Proliferative Markers and Neural Stem Cells Markers in Tanycytes of the Third Cerebral Ventricle in Rats.Bulletin of experimental biology and medicine · 2023Article
- Delayed Impairment of Hippocampal Synaptic Plasticity after Pentylenetetrazole-Induced Seizures in Young Rats.International journal of molecular sciences · 2022Article
- Methodology and Neuromarkers for Cetaceans' Brains.Veterinary sciences · 2022Article
- Changes in cytoplasmic and extracellular neuromelanin in human substantia nigra with normal aging.European journal of histochemistry : EJH · 2021Article
- Fluorescent characterization of amyloid deposits in the kidneys of mdx mice.European journal of histochemistry : EJH · 2018Article
- Histochemistry today: Detection and location of single molecules.European journal of histochemistry : EJH · 2017Article
- Is there still room for novelty, in histochemical papers?European journal of histochemistry : EJH · 2016Review
- Histochemistry in biology and medicine: a message from the citing journals.European journal of histochemistry : EJH · 2015Review
- The Spatial Organization of the Intranuclear Structures of Human Brain Dopaminergic Neurons.Acta naturaeArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue fixation is critical for immunohistochemistry. Recently, we developed a zinc-ethanol-formalin fixative (ZEF), and the present study was aimed to assess the applicability of the ZEF for the human brain histology and immunohistochemistry and to evaluate the detectability of different antigens in the human brain fixed with ZEF. In total, 11 antigens were tested, including NeuN, neuron-specific enolase, GFAP, Iba-1, calbindin, calretinin, choline acetyltransferase, glutamic acid decarboxylase (GAD65), tyrosine hydroxylase, synaptophysin, and α-tubulin. The obtained data show that: i) the ZEF has potential for use in general histological practice, where detailed characterization of human brain morphology is needed; ii) the antigens tested are well-preserved in the human brain specimens fixed in the ZEF.
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