ArticleDevelopment (Cambridge, England)2015
Intracellular lumen formation in Drosophila proceeds via a novel subcellular compartment.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- The biosynthetic-secretory pathway, supplemented by recycling routes, determines epithelial membrane polarity.Science advances · 2023Article
- Article
- An endosome-associated actin network involved in directed apical plasma membrane growth.The Journal of cell biology · 2022Article
- High-precision targeting workflow for volume electron microscopy.The Journal of cell biology · 2021Article
- Article
- Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism.The EMBO journal · 2020Article
- An Ichor-dependent apical extracellular matrix regulates seamless tube shape and integrity.PLoS genetics · 2018Article
- Time to make the doughnuts: Building and shaping seamless tubes.Seminars in cell & developmental biology · 2017Review
- Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae.Scientific reports · 2017Article
- Mapping the molecular steps of secretory-lysosome-driven tracheal tube fusion.Nature cell biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cellular tubes have diverse morphologies, including multicellular, unicellular and subcellular architectures. Subcellular tubes are found prominently within the vertebrate vasculature, the insect breathing system and the nematode excretory apparatus, but how such tubes form is poorly understood. To characterize the cellular mechanisms of subcellular tube formation, we have refined methods of high pressure freezing/freeze substitution to prepare Drosophila larvae for transmission electron microscopic (TEM) analysis. Using our methods, we have found that subcellular tube formation may proceed through a previously undescribed multimembrane intermediate composed of vesicles bound within a novel subcellular compartment. We have also developed correlative light/TEM procedures to identify labeled cells in TEM-fixed larval samples. Using this technique, we have found that Vacuolar ATPase (V-ATPase) and the V-ATPase regulator Rabconnectin-3 are required for subcellular tube formation, probably in a step resolving the intermediate compartment into a mature lumen. In general, our ultrastructural analysis methods could be useful for a wide range of cellular investigations in Drosophila larvae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.