Evidence map›Paper›PMID 26427713›Full record

ArticleIn vitro cellular & developmental biology. Animal2015

In vitro differentiation process of human Wharton's jelly mesenchymal stem cells to male germ cells in the presence of gonadal and non-gonadal conditioned media with retinoic acid.

Fardin Amidi, Nahid Ataie Nejad, Marziyeh Agha Hoseini, Karim Nayernia, Zohreh Mazaheri, Nazila Yamini, Sara Saeednia

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Article in In vitro cellular & developmental biology. Animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 2 pooled it
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 2 syntheses or guidelines pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Fardin AmidiDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, P.O. Box 13145-784, Tehran, Iran. Famidi@sina.tums.ac.ir.
Nahid Ataie NejadDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, P.O. Box 13145-784, Tehran, Iran.
Marziyeh Agha HoseiniDepartment of Obstetrics and Gynecology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Karim NayerniaInstitute for Molecular Medicine and Cell Therapy, Düsseldorf, Germany and GENEOCELL, Advanced Molecular & Cellular Technologies, Tehran, Iran.
Zohreh MazaheriDepartment of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Nazila YaminiDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, P.O. Box 13145-784, Tehran, Iran.
Sara SaeedniaDepartment of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Tehran University of Medical Sciences · IRSemnan University of Medical Sciences · IRStem Cell Technology Research Center · IRTarbiat Modares University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human umbilical Wharton's jelly-derived mesenchymal stem cells (HWJMSCs) are the best candidate to get plentiful stem cells and differentiate them to germ cells under appropriate conditions to treat infertility. We sought to determine under which conditions HWJMSCs could form male germ cells in vitro. So, HWJMSCs were differentiated to male germ cells under a mixture of bone morphogenetic protein-4 (BMP-4) and testicular and placental culture condition (TCC and PCC) medium followed by retinoic acid for 21 d. In the present study, the HWJMSCs were obtained from Wharton's jelly of umbilical cords of male neonates delivered by cesarean section. At the third passage, mesenchymal stem cell markers and differentiation to osteocytes and adipocytes were investigated. Then, HWJMSCs were induced to differentiate into male germ cells in the presence of BMP-4, all-trans retinoic acid, PCC, and TCC for 21 d. The profile of c-Kit, DDX4, Piwil2, and Dazl gene expression was evaluated by qPCR and ICC. Data was analyzed by ANOVA test. After 3 wk of treatment with different reagents, the morphology of these spindle-like cells changed to shiny clusters and germ cell-specific markers in mRNA were upregulated in both TCC + retinoic acid (RA) and BMP-4 + RA. Induction of HWJMSCs with TCC in the presence of RA resulted in significant upregulation (P ≤ 0.05) of all germ cell-specific genes (c-Kit 2.6795 ± 0.75, DDX4 4.3188 ± 1.18, Piwil2 4.9962 ± 1.55, Dazl 6.1199 ± 0.78) compared to control and PCC + RA. Our results indicated that TCC and RA are involved in human germ cell development. Moreover, BMP signaling also induced differentiation. Our findings provide a novel effective approach for generation of germ cells in vitro and studying the interaction of germ cells with their niche. Our work represents an essential step toward gaining knowledge of the molecular properties of HWJMSCs in the field of cell therapy. We demonstrated that under a suitable situation, HWJMSCs have the ability to differentiate into germ cells and this provides an excellent pattern to study infertility cause and cure.

Indexed as

Argonaute ProteinsBone Morphogenetic Protein 4Cell DifferentiationCulture Media, ConditionedDEAD-box RNA HelicasesFemaleHumansMaleMesenchymal Stem CellsPlacentaPolymerase Chain ReactionPregnancyProto-Oncogene Proteins c-kitRNA-Binding ProteinsRNA, MessengerSignal TransductionArgonaute ProteinsBMP4 protein, humanBone Morphogenetic Protein 4Culture Media, ConditionedDAZL protein, humanDDX4 protein, humanDEAD-box RNA HelicasesPIWIL2 protein, humanProto-Oncogene Proteins c-kitRNA-Binding ProteinsRNA, MessengerTissue ExtractsTretinoinBMP-4DifferentiationFetal testisGerm cellsMesenchymal stem cellsPlacentaRetinoic acidWharton’s jelly

Identifiers

PMID26427713
OpenAlexW1858046548

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.