ArticleIn vitro cellular & developmental biology. Animal2016
Development of primary cell cultures using hemocytes and phagocytic tissue cells of Locusta migratoria: an application for locust immunity studies.
Article in In vitro cellular & developmental biology. Animal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- A Straightforward Method for the Isolation and Cultivation ofInternational journal of molecular sciences · 2022Article
- Haemocyte-mediated immunity in insects: Cells, processes and associated components in the fight against pathogens and parasites.Immunology · 2021Review
- Haemocyte variations in 35 species of grasshoppers and locusts.Science progress · 2021Article
- Review
- A simple method for ex vivo honey bee cell culture capable of in vitro gene expression analysis.PloS one · 2021Article
- Hemocyte-hemocyte adhesion by granulocytes is associated with cellular immunity in the cricket, Gryllus bimaculatus.Scientific reports · 2019Article
- Cellular response to bacterial infection in the grasshopperBiology open · 2019Article
- Immune function of an angiotensin-converting enzyme against Rice stripe virus infection in a vector insect.Virology · 2019Article
- Hemocyte Morphology and Cellular Immune Response in Termite (Reticulitermes speratus).Journal of insect science (Online) · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect cell cultures played central roles in unraveling many insect physiological and immunological processes. Regardless, despite imminent needs, insect cell lines were developed primarily from Dipteran and Lepidopteran orders, leaving many important insects such as Orthopteran locusts under-represented. Besides the lack of cell lines, the slow progress in development of in vitro techniques is attributed to poor communications between different laboratories regarding optimized primary cell cultures. Therefore, we report here about methods developed for primary cell culture of Locusta migratoria hemocyte and phagocytic tissue cells by which we could maintain viable hemocytes in vitro for over 5 d and phagocytic tissue cells for over 12 d. 2-Mercaptoethanol and phenyl-thiourea supplements in Grace's medium together with addition of fetal bovine serum 30 min after cell seeding resulted in a successful setup of the primary cell cultures and a week-long survival of the hemocytes and phagocytic tissue cells in vitro.
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Registered trials
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