ArticleParasites & vectors2015
Development of a framework for genotyping bovine-derived Cryptosporidium parvum, using a multilocus fragment typing tool.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Understanding theFrontiers in parasitology · 2024Review
- Differences in virulence and oocyst shedding profiles in lambs experimentally infected with different isolates ofCurrent research in parasitology & vector-borne diseases · 2023Article
- Validation of a multilocus genotyping scheme for subtypingFood and waterborne parasitology · 2022Article
- Long-term production effects of clinical cryptosporidiosis in neonatal calves.International journal for parasitology · 2020Article
- A One Health Approach to Tackle Cryptosporidiosis.Trends in parasitology · 2020Review
- Microsatellite Analysis of Geographically Close Isolates ofFrontiers in veterinary science · 2019Article
- Public health risks associated with food-borne parasites.EFSA journal. European Food Safety Authority · 2018Article
- Genomic Variation in IbA10G2 and Other Patient-Derived Cryptosporidium hominis Subtypes.Journal of clinical microbiology · 2017Article
- Intra-Species Diversity and Panmictic Structure of Cryptosporidium parvum Populations in Cattle Farms in Northern Spain.PloS one · 2016Article
- Article
- Molecular epidemiologic tools for waterborne pathogensFood and waterborne parasitologyReview
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundThere is a need for an integrated genotyping approach for C. parvum; no sufficiently discriminatory scheme to date has been fully validated or widely adopted by veterinary or public health researchers. Multilocus fragment typing (MLFT) can provide good differentiation and is relatively quick and cheap to perform. A MLFT tool was assessed in terms of its typeability, specificity, precision (repeatability and reproducibility), accuracy and ability to genotypically discriminate bovine-derived Cryptosporidium parvum.
methodsWith the aim of working towards a consensus, six markers were selected for inclusion based on their successful application in previous studies: MM5, MM18, MM19, TP14, MS1 and MS9. Alleles were assigned according to the fragment sizes of repeat regions amplified, as determined by capillary electrophoresis. In addition, a region of the GP60 gene was amplified and sequenced to determine gp60 subtype and this was added to the allelic profiles of the 6 markers to determine the multilocus genotype (MLG). The MLFT tool was applied to 140 C. parvum samples collected in two cross-sectional studies of UK calves, conducted in Cheshire in 2004 (principally dairy animals) and Aberdeenshire/Caithness in 2011 (beef animals).
resultsTypeability was 84 %. The primers did not amplify tested non-parvum species frequently detected in cattle. In terms of repeatability, within- and between-run fragment sizes showed little variability. Between laboratories, fragment sizes differed but allele calling was reproducible. The MLFT had good discriminatory ability (Simpson's Index of Diversity, SID, was 0.92), compared to gp60 sequencing alone (SID 0.44). Some markers were more informative than others, with MS1 and MS9 proving monoallelic in tested samples.
conclusionsFurther inter-laboratory trials are now warranted with the inclusion of human-derived C. parvum samples, allowing progress towards an integrated, standardised typing scheme to enable source attribution and to determine the role of livestock in future outbreaks of human C. parvum.
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