ArticleeLife2015
Hypocretin neuron-specific transcriptome profiling identifies the sleep modulator Kcnh4a.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 63 citations in OpenAlex.
- Evolutionary Analysis of Vertebrate KCNH Voltage-Gated Potassium Channels and Spatial Expression ofJournal of developmental biology · 2026Article
- Evolutionary analysis of vertebrate KCNH voltage-gated potassium channels and their expression in zebrafish embryos.bioRxiv : the preprint server for biology · 2026Article
- Repeated Morphine Exposure Reversibly Impairs Spike Generation and Repetitive Firing in a Functionally Distinct Subpopulation of Orexin Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Modelling orexinergic system in ageing in the African turquoise killifish.Biogerontology · 2025Review
- Voltage Gated Ion Channels and Sleep.The Journal of membrane biology · 2024Review
- Single-Cell Profiling Uncovers Evolutionary Divergence of Hypocretin/Orexin Neuronal Subpopulations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Dorsoventral Arrangement of Lateral Hypothalamus Populations in the Mouse Hypothalamus: a Prosomeric Genoarchitectonic Analysis.Molecular neurobiology · 2023Article
- From Molecule to Behavior: Hypocretin/orexin Revisited From a Sex-dependent Perspective.Endocrine reviews · 2022Review
- Genetic and Neurological Deficiencies in the Visual System ofInternational journal of molecular sciences · 2022Article
- Large-scale Analysis of Sleep in Zebrafish.Bio-protocol · 2022Article
- Hypothalamic orexinergic neuron changes during the hibernation of the Syrian hamster.Frontiers in neuroanatomy · 2022Article
- Validation of Candidate Sleep Disorder Risk Genes Using Zebrafish.Frontiers in molecular neuroscience · 2022Review
- Transiently Nav1.8-expressing neurons are capable of sensing noxious stimuli in the brain.Frontiers in cellular neuroscience · 2022Article
- Recent advances in sleep genetics.Current opinion in neurobiology · 2021Review
- Article
- Heterogeneity of Hypocretin/Orexin Neurons.Frontiers of neurology and neuroscience · 2021Review
- Article
- A circuit perspective on narcolepsy.Sleep · 2020Article
- Maternal ethanol consumption before paternal fertilization: Stimulation of hypocretin neurogenesis and ethanol intake in zebrafish offspring.Progress in neuro-psychopharmacology & biological psychiatry · 2020Article
- Neurogenetic basis for circadian regulation of metabolism by the hypothalamus.Genes & development · 2019Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sleep has been conserved throughout evolution; however, the molecular and neuronal mechanisms of sleep are largely unknown. The hypothalamic hypocretin/orexin (Hcrt) neurons regulate sleep\wake states, feeding, stress, and reward. To elucidate the mechanism that enables these various functions and to identify sleep regulators, we combined fluorescence cell sorting and RNA-seq in hcrt:EGFP zebrafish. Dozens of Hcrt-neuron-specific transcripts were identified and comprehensive high-resolution imaging revealed gene-specific localization in all or subsets of Hcrt neurons. Clusters of Hcrt-neuron-specific genes are predicted to be regulated by shared transcription factors. These findings show that Hcrt neurons are heterogeneous and that integrative molecular mechanisms orchestrate their diverse functions. The voltage-gated potassium channel Kcnh4a, which is expressed in all Hcrt neurons, was silenced by the CRISPR-mediated gene inactivation system. The mutant kcnh4a (kcnh4a(-/-)) larvae showed reduced sleep time and consolidation, specifically during the night, suggesting that Kcnh4a regulates sleep.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.