Evidence map›Paper›PMID 26426328›Full record

ArticlePloS one2015

Correlation between Serum Levels of 3,3',5'-Triiodothyronine and Thyroid Hormones Measured by Liquid Chromatography-Tandem Mass Spectrometry and Immunoassay.

Hiroyuki Sakai, Hidenori Nagao, Mamoru Sakurai, Takako Okumura, Yoshiyuki Nagai, Junpei Shikuma, Rokuro Ito, Tetsuya Imazu, Takashi Miwa, Masato Odawara

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 18 citations in OpenAlex.

  1. Biological Applications for LC-MS-Based Proteomics.Advances in experimental medicine and biology · 2021
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  2. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Hiroyuki SakaiDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Hidenori NagaoPharmacokinetics Research Department of ASKA Pharmaceutical Co., Ltd., Kawasaki, Kanagawa, Japan.
Mamoru SakuraiDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Takako OkumuraDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Yoshiyuki NagaiDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Junpei ShikumaDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Rokuro ItoDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Tetsuya ImazuPharmacokinetics Research Department of ASKA Pharmaceutical Co., Ltd., Kawasaki, Kanagawa, Japan.
Takashi MiwaDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Masato OdawaraDepartment of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
Tokyo Medical University · JPASKA Pharmaceutical (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveFor measuring serum 3,3',5'-triiodothyronine (rT3) levels, radioimmunoassay (RIA) has traditionally been used owing to the lack of other reliable methods; however, it has recently become difficult to perform. Meanwhile, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has recently been attracting attention as a novel alternative method in clinical chemistry. To the best of our knowledge, there are no studies to date comparing results of the quantification of human serum rT3 between LC-MS/MS and RIA. We therefore examined the feasibility of LC-MS/MS as a novel alternative method for measuring serum rT3, thyroxine (T4), and 3,5,3'-triiodothyronine (T3) levels.

methodsAssay validation was performed by LC-MS/MS using quality control samples of rT3, T4, and T3 at 4 various concentrations which were prepared from reference compounds. Serum samples of 50 outpatients in our department were quantified both by LC-MS/MS and conventional immunoassay for rT3, T4, and T3. Correlation coefficients between the 2 measurement methods were statistically analyzed respectively.

resultsMatrix effects were not observed with our method. Intra-day and inter-day precisions were less than 10.8% and 9.6% for each analyte at each quality control level, respectively. Intra-day and inter-day accuracies were between 96.2% and 110%, and between 98.3% and 108.6%, respectively. The lower limit of quantification was 0.05 ng/mL. Strong correlations were observed between the 2 measurement methods (correlation coefficient, T4: 0.976, p < 0.001; T3: 0.912, p < 0.001; rT3: 0.928, p < 0.001).

conclusionsOur LC-MS/MS system requires no manual cleanup operation, and the process after application of a sample is fully automated; furthermore, it was found to be highly sensitive, and superior in both precision and accuracy. The correlation between the 2 methods over a wide range of concentrations was strong. LC-MS/MS is therefore expected to become a useful tool for clinical diagnosis and research.

Indexed as

Blood Chemical AnalysisChromatography, LiquidFeasibility StudiesFemaleHumansLinear ModelsMaleMiddle AgedRadioimmunoassayReproducibility of ResultsTandem Mass SpectrometryThyroxineTriiodothyronineTriiodothyronine, ReverseThyroxineTriiodothyronineTriiodothyronine, Reverse

Identifiers

PMID26426328
PMCPMC4591014
OpenAlexW2266922729

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.