Evidence map›Paper›PMID 26425808›Full record

ArticleEndocrinology2015

Using a 3D Culture System to Differentiate Visceral Adipocytes In Vitro.

Margo P Emont, Hui Yu, Heejin Jun, Xiaowei Hong, Nenita Maganti, Jan P Stegemann, Jun Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Trial
  2. Article
  3. Review
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  5. Review
  6. Review
  7. Integrated analysis of the tumor microenvironment using a reconfigurable microfluidic cell culture platform.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Review
  16. BMC biomedical engineering · 2019
    Article
  17. Article
  18. Bone: Bone marrow adipocytes in 3D.Nature reviews. Endocrinology · 2018
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Margo P EmontLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.
Hui YuLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.
Heejin JunLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.
Xiaowei HongLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.
Nenita MagantiLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.
Jan P StegemannLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.
Jun WuLife Sciences Institute (M.P.E., H.Y., H.J., J.W.), Departments of Molecular and Integrative Physiology (M.P.E., J.W.) and Biomedical Engineering (X.H., J.P.S.), and College of Literature, Science, and the Arts (N.M.), University of Michigan, Ann Arbor, Michigan 48109.

Funding

Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID P OLSON · 2013 to 2026
$24.3M
Pilot and Feasibility (P and F) ProgramP30DK089503 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RANDY J SEELEY · 2010 to 2026
$20.3M
Molecular Regulation of Beige Fat Cells in Mice and HumansR03DK100698 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WU, JUN · 2014 to 2015
$155k
NIDDK NIH HHS 2P30-DK020572NIDDK NIH HHS P30 DK020572NIDDK NIH HHS P30 DK089503NIDDK NIH HHS P30-DK089503NIDDK NIH HHS R03 DK100698NIDDK NIH HHS R03DK100698
6 · The paper itself

Abstract

It has long been recognized that body fat distribution and regional adiposity play a major role in the control of metabolic homeostasis. However, the ability to study and compare the cell autonomous regulation and response of adipocytes from different fat depots has been hampered by the difficulty of inducing preadipocytes isolated from the visceral depot to differentiate into mature adipocytes in culture. Here, we present an easily created 3-dimensional (3D) culture system that can be used to differentiate preadipocytes from the visceral depot as robustly as those from the sc depot. The cells differentiated in these 3D collagen gels are mature adipocytes that retain depot-specific characteristics, as determined by imaging, gene expression, and functional assays. This 3D culture system therefore allows for study of the development and function of adipocytes from both depots in vitro and may ultimately lead to a greater understanding of site-specific functional differences of adipose tissues to metabolic dysregulation.

Indexed as

Cell DifferentiationCollagenHydrogelsAdipocytesAnimalsBlotting, WesternCell Culture TechniquesEnzyme-Linked Immunosorbent AssayGene ExpressionIntra-Abdominal FatIn Vitro TechniquesMiceReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStem CellsSubcutaneous FatCollagenHydrogelsRNA, Messenger

Identifiers

PMID26425808
PMCPMC4655212

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.