ArticleCartilage2015
In Vitro Toxicity of Local Anesthetics and Corticosteroids on Chondrocyte and Synoviocyte Viability and Metabolism.
Article in Cartilage, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Is arthrocentesis of temporomandibular joint with corticosteroids beneficial? A systematic review.Medicina oral, patologia oral y cirugia bucal · 2018Pooled it
- Single-dose local anesthetics exhibit a type-, dose-, and time-dependent chondrotoxic effect on chondrocytes and cartilage: a systematic review of the current literature.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2018Pooled it
- Clinical efficacy of intra-articular lidocaine with or without adrenaline during shoulder arthroscopy in dogs.Frontiers in veterinary science · 2026Trial
- In Vitro Cytotoxicity and Inflammatory Response of Local Anaesthetics and Corticosteroids in Canine Fibroblast-Like Synoviocytes.Cartilage · 2026Article
- Optimizing Osteoarthritis Management - Therapeutic Efficacy and Evaluation of Intra-Articular Triamcinolone Acetonide Injection.Journal of pain research · 2026Review
- A survey of the clinical usage of intra-articular therapeutics in dogs by veterinary practitioners with a focus on non-steroidal therapies.Frontiers in veterinary science · 2026Article
- Revisiting the Chondrotoxicity of Intra-Articular Triamcinolone Acetonide in Osteoarthritis: Dose Dependence and Experimental Model Heterogeneity [Letter].Journal of pain research · 2026Article
- Autologous Peripheral Blood-Derived Orthobiologics for the Management of Shoulder Disorders: A Review of Current Clinical Evidence.Pain and therapy · 2025Review
- The Combined Use of Triamcinolone and Platelet-Rich Plasma in Equine Metacarpophalangeal Joint Osteoarthritis Treatments: An In Vivo and In Vitro Study.Animals : an open access journal from MDPI · 2024Article
- Advanced Non-Operative Interventions for Anterior Knee Pain.Current reviews in musculoskeletal medicine · 2024Review
- Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Intra-articular corticosteroids for the treatment of osteoarthritis: A systematic review and meta-analysis on the comparison of different molecules and doses.Journal of experimental orthopaedics · 2024Review
- Chondrotoxicity of Intra-Articular Injection Treatment: A Scoping Review.International journal of molecular sciences · 2024Article
- Rapid Osteoarthritis and Femoral Head Collapse: A Case Series.Journal of orthopaedic case reports · 2024Article
- The Combination of Glucocorticoids and Hyaluronic Acid Enhances Efficacy in IL-1β/IL-17-Treated Bovine Osteochondral Grafts Compared with Individual Application.International journal of molecular sciences · 2023Article
- Treatment Options for Patellofemoral Arthritis.Current reviews in musculoskeletal medicine · 2022Review
- Article
- Local Anesthetics' Toxicity toward Human Cultured Chondrocytes: A Comparative Study between Lidocaine, Bupivacaine, and Ropivacaine.Cartilage · 2019Article
- Cytotoxicity of drugs injected into joints in orthopaedics.Bone & joint research · 2019Article
- Effect of a single intra-articular injection of bupivacaine on synovial fluid prostaglandin EBMC research notes · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThere is growing concern that intra-articular injection of local anesthetic and/or corticosteroids may cause significant morbidity, including potential toxicity to chondrocytes and synoviocytes, after even a single exposure. We demonstrate that full thickness canine chondral and synovial samples exposed to various local anesthetics and corticosteroids exhibit decreased loss of cell viability compared with prior in vitro studies using monolayer culture, due to the protective effects of intact extracellular matrix and cell heterogeneity.
methodsFull-thickness cartilage and synovial explants were obtained from canine cadavers and exposed in culture media to the following for 24 hours: 1% lidocaine, 0.5% lidocaine, 0.25% bupivacaine, 0.125% bupvacaine, 0.0625% bupivacaine, betamethasone acetate, methylprednisolone acetate, triamcinolone acetonide, or culture media only (control). Cell viability was determined on days 1 and 7 of culture using a microscopic live-dead and alamar blue metabolic assays.
resultsComplete loss of chondrocyte and synoviocyte viability was noted in the 1% and 0.5% lidocaine group, 0.25% and 0.125% bupivacaine group, betamethasone group, and methylprednisolone groups after 1 and 7 days of culture. Treatment with 0.0625% bupivacaine and triamcinolone demonstrated no decrease in cell viability or metabolism when compared to negative control.
conclusionsIn this canine explant model, 1% and 0.5% lidocaine, 0.25% and 0.125% bupivacaine, betamethasone acetate, and methylpresdnisolone acetate were severely chondrotoxic and synoviotoxic after a single exposure, despite intact extracellular matrix. In contrast, chondrocytes and synoviocytes exposed to 0.0625% bupivacaine and triamcinolone remained viable after treatment. Further in vivo study is needed before definitive recommendations can be made.
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Registered trials
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