ArticleJournal of clinical microbiology2015
Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 124 citations in OpenAlex.
- Research progress into the principles and methods underlying capsular typing of Glaesserella parasuis.Veterinary research · 2024Pooled it
- Minimum dose, antigen content, and immunization duration of a trivalent vaccine of inactivatedCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Trial
- Evidence for Genotype-Associated Differences in Disease Severity and Limitations of Serotype-Based Classification inPathogens (Basel, Switzerland) · 2026Article
- Article
- A Triplex Real-Time PCR Assay for Simultaneous Detection ofTransboundary and emerging diseases · 2026Article
- Establishment of a TaqMan multiplex quantitative PCR method for detecting three different serovars of Glaesserella parasuis serovars 5/12, 7 and 11.BMC veterinary research · 2025Article
- Isolation and identification of two highly virulent Glaesserella parasuis serovar 7 strains in China.BMC microbiology · 2025Article
- Pathotyping and molecular serotyping of clinical isolates of Glaesserella parasuis in Taiwan.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Comparison of antimicrobial susceptibility of Glaesserella parasuis from different pig production systems in Taiwan between 2015 and 2020.Porcine health management · 2025Article
- Article
- Epidemiological and comparative genomic analysis of pathogenicFrontiers in microbiology · 2025Article
- Genomic insights into diversity, antimicrobial resistance and virulence ofFrontiers in microbiology · 2025Article
- Genomic insight into the diversity ofmSphere · 2024Article
- EspP2 Regulates the Adhesion ofInternational journal of molecular sciences · 2024Article
- Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems.BMC veterinary research · 2023Article
- Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulentVaccine: X · 2023Article
- Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae.MicrobiologyOpen · 2023Article
- Molecular Characterization and Phylogenetic Analysis ofPathogens (Basel, Switzerland) · 2023Article
- Rapid Visual Detection ofTransboundary and emerging diseases · 2023Article
- Article
Corrections and comments
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Authors and funding
17 authors at 9 institutions in 3 countries.
Funding
Abstract
Haemophilus parasuis causes Glässer's disease and pneumonia in pigs. Indirect hemagglutination (IHA) is typically used to serotype this bacterium, distinguishing 15 serovars with some nontypeable isolates. The capsule loci of the 15 reference strains have been annotated, and significant genetic variation was identified between serovars, with the exception of serovars 5 and 12. A capsule locus and in silico serovar were identified for all but two nontypeable isolates in our collection of >200 isolates. Here, we describe the development of a multiplex PCR, based on variation within the capsule loci of the 15 serovars of H. parasuis, for rapid molecular serotyping. The multiplex PCR (mPCR) distinguished between all previously described serovars except 5 and 12, which were detected by the same pair of primers. The detection limit of the mPCR was 4.29 × 10(5) ng/μl bacterial genomic DNA, and high specificity was indicated by the absence of reactivity against closely related commensal Pasteurellaceae and other bacterial pathogens of pigs. A subset of 150 isolates from a previously sequenced H. parasuis collection was used to validate the mPCR with 100% accuracy compared to the in silico results. In addition, the two in silico-nontypeable isolates were typeable using the mPCR. A further 84 isolates were analyzed by mPCR and compared to the IHA serotyping results with 90% concordance (excluding those that were nontypeable by IHA). The mPCR was faster, more sensitive, and more specific than IHA, enabling the differentiation of 14 of the 15 serovars of H. parasuis.
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