ArticleMolecular biology of the cell2015
Probability-based particle detection that enables threshold-free and robust in vivo single-molecule tracking.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Optimized molecule detection in localization microscopy with selected false positive probability.Nature communications · 2025Article
- SOLEIL: single-objective lens inclined light sheet localization microscopy.Biomedical optics express · 2022Article
- Bayesian machine learning analysis of single-molecule fluorescence colocalization images.eLife · 2022Article
- Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adultFrontiers in neuroscience · 2022Article
- DoGNet: A deep architecture for synapse detection in multiplexed fluorescence images.PLoS computational biology · 2019Article
- Advanced 3D Analysis and Optimization of Single-Molecule FISH inSmall methods · 2018Article
- Molecular imaging with neural training of identification algorithm (neural network localization identification).Microscopy research and technique · 2018Article
- Variational Algorithms for Analyzing Noisy Multistate Diffusion Trajectories.Biophysical journal · 2018Article
- Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- TrackNTrace: A simple and extendable open-source framework for developing single-molecule localization and tracking algorithms.Scientific reports · 2016Article
- Automatic Bayesian single molecule identification for localization microscopy.Scientific reports · 2016Article
- Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.Journal of molecular biology · 2016Review
- Simultaneous measurement of emission color and 3D position of single molecules.Optics express · 2016Article
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5 authors.
Funding
Abstract
Single-molecule detection in fluorescence nanoscopy has become a powerful tool in cell biology but can present vexing issues in image analysis, such as limited signal, unspecific background, empirically set thresholds, image filtering, and false-positive detection limiting overall detection efficiency. Here we present a framework in which expert knowledge and parameter tweaking are replaced with a probability-based hypothesis test. Our method delivers robust and threshold-free signal detection with a defined error estimate and improved detection of weaker signals. The probability value has consequences for downstream data analysis, such as weighing a series of detections and corresponding probabilities, Bayesian propagation of probability, or defining metrics in tracking applications. We show that the method outperforms all current approaches, yielding a detection efficiency of >70% and a false-positive detection rate of <5% under conditions down to 17 photons/pixel background and 180 photons/molecule signal, which is beneficial for any kind of photon-limited application. Examples include limited brightness and photostability, phototoxicity in live-cell single-molecule imaging, and use of new labels for nanoscopy. We present simulations, experimental data, and tracking of low-signal mRNAs in yeast cells.
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