ArticleMolecular biology of the cell2015
Spontaneous and electric field-controlled front-rear polarization of human keratinocytes.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- SCHEPHERD: A modular, programmable, direct current platform to control cell behavior.Science advances · 2026Article
- Macrophages sense and follow physiologically relevant KCell communication and signaling : CCS · 2026Article
- Electrotaxis evokes directional separation of co-cultured keratinocytes and fibroblasts.Scientific reports · 2023Article
- Bioelectronic microfluidic wound healing: a platform for investigating direct current stimulation of injured cell collectives.Lab on a chip · 2023Article
- Bioelectric Signaling: Role of Bioelectricity in Directional Cell Migration in Wound Healing.Cold Spring Harbor perspectives in biology · 2022Review
- Cell-cell interactions and fluctuations in the direction of motility promote directed migration of osteoblasts in direct current electrotaxis.Frontiers in bioengineering and biotechnology · 2022Article
- Quantifying the impact of electric fields on single-cell motility.Biophysical journal · 2021Article
- Overriding native cell coordination enhances external programming of collective cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients.Frontiers in immunology · 2021Article
- Novel Electrode Designs for Neurostimulation in Regenerative Medicine: Activation of Stem Cells.Bioelectricity · 2020Review
- Substrate-Dependent Galvanotaxis of Directly Reprogrammed Human Neural Precursor Cells.Bioelectricity · 2020Article
- Bioelectrical controls of morphogenesis: from ancient mechanisms of cell coordination to biomedical opportunities.Current opinion in genetics & development · 2019Review
- Electric fields accelerate cell polarization and bypass myosin action in motility initiation.Journal of cellular physiology · 2018Article
- Environmental Factors That Influence Stem Cell Migration: An "Electric Field".Stem cells international · 2017Review
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Authors and funding
7 authors.
Funding
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Abstract
It has long been known that electrical fields (EFs) are able to influence the direction of migrating cells, a process commonly referred to as electrotaxis or galvanotaxis. Most studies have focused on migrating cells equipped with an existing polarity before EF application, making it difficult to delineate EF-specific pathways. Here we study the initial events in front-rear organization of spreading keratinocytes to dissect the molecular requirements for random and EF-controlled polarization. We find that Arp2/3-dependent protrusive forces and Rac1/Cdc42 activity were generally required for both forms of polarization but were dispensable for controlling the direction of EF-controlled polarization. By contrast, we found a crucial role for extracellular pH as well as G protein coupled-receptor (GPCR) or purinergic signaling in the control of directionality. The normal direction of polarization toward the cathode was reverted by lowering extracellular pH. Polarization toward the anode was also seen at neutral pH when GPCR or purinergic signaling was inhibited. However, the stepwise increase of extracellular pH in this scenario led to restoration of cathodal polarization. Overall our work puts forward a model in which the EF uses distinct polarization pathways. The cathodal pathway involves GPCR/purinergic signaling and is dominant over the anodal pathway at neutral pH.
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