ArticleScientific reports2015
Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- DNA Oxidation and Expression of Repair Enzymes in Organ- Cultured Human Limbal Epithelium.International journal of molecular sciences · 2026Article
- Stem Cell-Derived Corneal Epithelium: Engineering Barrier Function for Ocular Surface Repair.International journal of molecular sciences · 2025Review
- The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells.Frontiers in cell and developmental biology · 2025Article
- Single nuclei transcriptomics of the in situ human limbal stem cell niche.Scientific reports · 2024Article
- Knockout of c-Cbl slows EGFR endocytic trafficking and enhances EGFR signaling despite incompletely blocking receptor ubiquitylation.Pharmacology research & perspectives · 2021Article
- PFN1 and integrin-β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells.Journal of cellular and molecular medicine · 2019Article
- Application of corneal injury models in dual fluorescent reporter transgenic mice to understand the roles of the cornea and limbus in angiogenic and lymphangiogenic privilege.Scientific reports · 2019Article
- Corneal cell therapy: with iPSCs, it is no more a far-sight.Stem cell research & therapy · 2018Review
- Induced pluripotent stem cells as a potential therapeutic source for corneal epithelial stem cells.International journal of ophthalmology · 2018Review
- The Role of Limbal Epithelial Stem Cells in Regulating Corneal (Lymph)angiogenic Privilege and the Micromilieu of the Limbal Niche following UV Exposure.Stem cells international · 2018Review
- Comparison of iTRAQ and SWATH in a clinical study with multiple time points.Clinical proteomics · 2018Article
- Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium.Scientific reports · 2017Article
- Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes.PloS one · 2017Article
- Induced pluripotent stem cell-derived limbal epithelial cells (LiPSC) as a cellular alternative for in vitro ocular toxicity testing.PloS one · 2017Article
- Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells.PloS one · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Limbal epithelial stem cells (LESCs) are tissue-specific stem cells responsible for renewing the corneal epithelium. Acute trauma or chronic disease affecting LESCs may disrupt corneal epithelial renewal, causing vision threatening and painful ocular surface disorders, collectively referred to as LESC deficiency (LESCD). These disorders cannot be treated with traditional corneal transplantation and therefore alternative cell sources for successful cell-based therapy are needed. LESCs derived from human pluripotent stem cells (hPSCs) are a prospective source for ocular surface reconstruction, yet critical evaluation of these cells is crucial before considering clinical applications. In order to quantitatively evaluate hPSC-derived LESCs, we compared protein expression in native human corneal cells to that in hPSC-derived LESCs using isobaric tag for relative and absolute quantitation (iTRAQ) technology. We identified 860 unique proteins present in all samples, including proteins involved in cell cycling, proliferation, differentiation and apoptosis, various LESC niche components, and limbal and corneal epithelial markers. Protein expression profiles were nearly identical in LESCs derived from two different hPSC lines, indicating that the differentiation protocol is reproducible, yielding homogeneous cell populations. Their protein expression profile suggests that hPSC-derived LESCs are similar to the human ocular surface epithelial cells, and possess LESC-like characteristics.
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Registered trials
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