ArticleJournal of biomedical materials research. Part A2016
Injectable hydrogel provides growth-permissive environment for human nucleus pulposus cells.
Article in Journal of biomedical materials research. Part A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Effects of cell culture time and cytokines on migration of dental pulp stem cell-derived chondrogenic cells in collagen hydrogels.Physiological reports · 2024Article
- Intradiscal injection for the management of low back pain.JOR spine · 2022Article
- Development of 2-D and 3-D culture platforms derived from decellularized nucleus pulposus.Frontiers in bioengineering and biotechnology · 2022Article
- MR Imaging of Migrated Nucleus Pulposus after Collagenase Injection of Lumbar Disc Herniation: A Case Report.Journal of the Belgian Society of Radiology · 2022Article
- Collagen- and hyaluronic acid-based hydrogels and their biomedical applications.Materials science & engineering. R, Reports : a review journal · 2021Article
- Using embedded alginate microparticles to tune the properties of in situ forming poly(JOR spine · 2021Article
- Multiscale Regulation of the Intervertebral Disc: Achievements in Experimental, In Silico, and Regenerative Research.International journal of molecular sciences · 2021Review
- Endogenous repair theory enriches construction strategies for orthopaedic biomaterials: a narrative review.Biomaterials translational · 2021Review
- Review
- Preparation of an acellular spinal cord scaffold to improve its biological properties.Molecular medicine reports · 2019Article
- Core-Shell-Shell Nanoparticles for NIR Fluorescence Imaging and NRET Swelling Reporting of Injectable or Implantable Gels.Biomacromolecules · 2019Article
- Article
- Flow Behavior Prior to Crosslinking: The Need for Precursor Rheology for Placement of Hydrogels in Medical Applications and for 3D Bioprinting.Progress in polymer science · 2019Article
- Effect of optimized collagenase digestion on isolated and cultured nucleus pulposus cells in degenerated intervertebral discs.Medicine · 2018Observational
- Regeneration of sciatic nerve crush injury by a hydroxyapatite nanoparticle-containing collagen type I hydrogel.The journal of physiological sciences : JPS · 2018Article
- Dental pulp stem cell-derived chondrogenic cells demonstrate differential cell motility in type I and type II collagen hydrogels.The spine journal : official journal of the North American Spine Society · 2018Article
- Biomimetic nucleus pulposus scaffold created from bovine caudal intervertebral disc tissue utilizing an optimal decellularization procedure.Journal of biomedical materials research. Part A · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Degeneration of intervertebral discs (IVDs) results in an overall alteration of the biomechanics of the spinal column and becomes a major cause of low back pain. In this study, an injectable hydrogel composite is fabricated and characterized as a potential scaffold for the treatment of degenerated IVDs. Crosslinking of type II collagen-hyaluronic acid (HA) hydrogel with 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) increases the gel stability against collagenase digestion and reduces water uptake in comparison with non-crosslinked gel. Cell viability assay exhibits the proliferation of human nucleus pulposus (HNP) cells in hydrogels. The cells in non-crosslinked gel and the gel crosslinked with a low concentration of EDC (0.1 mM) show superior cell viability and morphology compared with cells in gels crosslinked with higher concentration of EDC. Quantitative PCR assay demonstrates the gene expression of extracellular matrix (ECM) by cells cultured in the gels. The expression of ECM genes by HNP cells in the gels demonstrated the phenotypic change of the cells. This study suggests that the type II collagen-HA hydrogel and crosslinked hydrogel (0.1 mM EDC) are permissive matrix for the growth of HNP cells and can be potentially applied in NP repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.