Evidence map›Paper›PMID 26421285›Full record

ArticleBioMed research international2015

Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells.

Fu-Lan Yang, Fang-Zhi Chen, Xin-Xing Wan, Xi Zhou, Mei-Juan Zhou, Han-Chun Chen, Jun-Jiang Fu, Dian-Zheng Zhang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Fu-Lan YangDepartment of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
Fang-Zhi ChenDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
Xin-Xing WanDepartment of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
Xi ZhouDepartment of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
Mei-Juan ZhouDepartment of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
Han-Chun ChenDepartment of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
Jun-Jiang FuKey Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou, Sichuan 646000, China.
Dian-Zheng ZhangDepartment of Biochemistry/Molecular Biology, Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, USA.
Central South University · CNFirst Affiliated Hospital of Sichuan Medical University · CNPhiladelphia College of Osteopathic Medicine · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using the phage display biopanning technique, we have previously identified a heptapeptide KLWVIPQ which specifically binds to the surface of the IFN-α-sensitive but not the IFN-α-resistant CML cells. The effects of this heptapeptide on the IFN-α-sensitive CML cells were investigated in the present study. IFN-α-sensitive KT-1/A3 and IFN-α-resistant KT-1/A3R CML cells were transfected by pEGFP-KLWVIPQ expression vector and/or induced by IFN-α. WST-1 cell proliferation assay, flow cytometry, and western blotting were performed to determine the effects of this heptapeptide and/or IFN-α on CML cells. The viability of the KT-1/A3 cells was inhibited and apoptosis was induced by either expression of the heptapeptide KLWVIPQ or IFN-α treatment with concurrent upregulation of P53 and downregulation of P210(bcr/abl). However, these effects were not observed in the IFN-α-resistant KT-1/A3R cells. These results suggest that the heptapeptide KLWVIPQ shares a similar mechanism with IFN-α in the regulation of CML cell growth and apoptosis, implying that the heptapeptide KLWVIPQ could be a novel target to go further into mechanisms of IFN-α sensitivity and/or resistance in CML.

Indexed as

Amino Acid SequenceCell Line, TumorCell SurvivalGene Expression Regulation, LeukemicGenetic VectorsGreen Fluorescent ProteinsHumansInterferon-alphaLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMolecular Sequence DataOligopeptidesPlasmidsRecombination, GeneticTransfectionTumor Suppressor Protein p53enhanced green fluorescent proteinGreen Fluorescent ProteinsInterferon-alphaOligopeptidesTumor Suppressor Protein p53

Identifiers

PMID26421285
PMCPMC4569753
OpenAlexW1870571582

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.