Evidence map›Paper›PMID 26419778›Full record

ArticlePlant physiology2015

Ectopic Expression of WRINKLED1 Affects Fatty Acid Homeostasis in Brachypodium distachyon Vegetative Tissues.

Yang Yang, Jacob Munz, Cynthia Cass, Agnieszka Zienkiewicz, Que Kong, Wei Ma, Sanjaya, John Sedbrook, Christoph Benning

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed, 1 pooled it
5.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.

  1. Pooled it
  2. Review
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  20. Overexpression of SoybeanInternational journal of molecular sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Yang YangDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Jacob MunzDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Cynthia CassDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Agnieszka ZienkiewiczDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Que KongDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Wei MaDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Sanjaya
John SedbrookDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.).
Christoph BenningDepartment of Biochemistry and Molecular Biology (Y.Y., A.Z., Q.K., C.B.), Great Lakes Bioenergy Research Center (Y.Y., J.M., C.C., A.Z., Q.K., W.M., S., J.S., C.B.), and Department of Plant Biology (W.M.), Michigan State University, East Lansing, Michigan 48824;School of Biological Sciences, Illinois State University, Normal, Illinois 61790 (J.M., C.C., J.S.); andMichigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824 (Y.Y., A.Z., Q.K., W.M., C.B.) benning@msu.edu.ORCID http://orcid.org/0000-0001-8585-3667
Great Lakes Bioenergy Research Center · USMichigan State University · USIllinois State University · USWest Virginia State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Triacylglycerol (TAG) is a storage lipid used for food purposes and as a renewable feedstock for biodiesel production. WRINKLED1 (WRI1) is a transcription factor that governs fatty acid (FA) synthesis and, indirectly, TAG accumulation in oil-storing plant tissues, and its ectopic expression has led to TAG accumulation in vegetative tissues of different dicotyledonous plants. The ectopic expression of BdWRI1 in the grass Brachypodium distachyon induced the transcription of predicted genes involved in glycolysis and FA biosynthesis, and TAG content was increased up to 32.5-fold in 8-week-old leaf blades. However, the ectopic expression of BdWRI1 also caused cell death in leaves, which has not been observed previously in dicotyledonous plants such as Arabidopsis (Arabidopsis thaliana). Lipid analysis indicated that the free FA content was 2-fold elevated in BdWRI1-expressing leaf blades of B. distachyon. The transcription of predicted genes involved in β-oxidation was induced. In addition, linoleic FA treatment caused cell death in B. distachyon leaf blades, an effect that was reversed by the addition of the FA biosynthesis inhibitor cerulenin. Taken together, ectopic expression of BdWRI1 in B. distachyon enhances FA biosynthesis and TAG accumulation in leaves, as expected, but also leads to increased free FA content, which has cytotoxic effects leading to cell death. Thus, while WRI appears to ubiquitously affect FA biosynthesis and TAG accumulation in diverse plants, its ectopic expression can lead to undesired side effects depending on the context of the specific lipid metabolism of the respective plant species.

Indexed as

BrachypodiumEctopic Gene ExpressionFatty AcidsGene Expression Regulation, PlantHomeostasisLipidsPlant OilsPlant ProteinsSeedsTranscription FactorsTriglyceridesFatty AcidsLipidsPlant OilsPlant ProteinsTranscription FactorsTriglycerides

Identifiers

PMID26419778
PMCPMC4634098
OpenAlexW1906322448

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.