ArticleMolecular microbiology2016
PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroides.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular dissection of the Cereibacter sphaeroides spheroidene monooxygenase.The Biochemical journal · 2026Article
- Differential stability of bacterial photosynthetic apparatus ofFrontiers in microbiology · 2024Article
- Article
- Atomic force microscopic analysis of the light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum.Photosynthesis research · 2023Article
- The structure and assembly of reaction centre-light-harvesting 1 complexes in photosynthetic bacteria.Bioscience reports · 2023Article
- FtsH4 protease controls biogenesis of the PSII complex by dual regulation of high light-inducible proteins.Plant communications · 2023Article
- Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic PhototrophicMicroorganisms · 2022Article
- The terminal enzymes of (bacterio)chlorophyll biosynthesis.Royal Society open science · 2022Review
- Cryo-EM Structure of theBiochemistry · 2021Article
- Biosynthesis of the modified tetrapyrroles-the pigments of life.The Journal of biological chemistry · 2020Review
- Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna.Biochimica et biophysica acta. Bioenergetics · 2019Article
- Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase.FEBS letters · 2018Article
- Carotenoid to bacteriochlorophyll energy transfer in the RC-LH1-PufX complex from Rhodobacter sphaeroides containing the extended conjugation keto-carotenoid diketospirilloxanthin.Photosynthesis research · 2018Article
- Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex.Biochimica et biophysica acta. Bioenergetics · 2018Article
- PufQ regulates porphyrin flux at the haem/bacteriochlorophyll branchpoint of tetrapyrrole biosynthesis via interactions with ferrochelatase.Molecular microbiology · 2017Article
- Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting.Biochimica et biophysica acta. Bioenergetics · 2017Article
- Mapping the ultrafast flow of harvested solar energy in living photosynthetic cells.Nature communications · 2017Article
- Heterologous Production of the Photosynthetic Reaction Center and Light Harvesting 1 Complexes of the Thermophile Thermochromatium tepidum in the Mesophile Rhodobacter sphaeroides and Thermal Stability of a Hybrid Core Complex.Applied and environmental microbiology · 2017Article
- The C-terminus of PufX plays a key role in dimerisation and assembly of the reaction center light-harvesting 1 complex from Rhodobacter sphaeroides.Biochimica et biophysica acta. Bioenergetics · 2017Article
- The PufX quinone channel enables the light-harvesting 1 antenna to bind more carotenoids for light collection and photoprotection.FEBS letters · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The mature architecture of the photosynthetic membrane of the purple phototroph Rhodobacter sphaeroides has been characterised to a level where an atomic-level membrane model is available, but the roles of the putative assembly proteins LhaA and PucC in establishing this architecture are unknown. Here we investigate the assembly of light-harvesting LH2 and reaction centre-light-harvesting1-PufX (RC-LH1-PufX) photosystem complexes using spectroscopy, pull-downs, native gel electrophoresis, quantitative mass spectrometry and fluorescence lifetime microscopy to characterise a series of lhaA and pucC mutants. LhaA and PucC are important for specific assembly of LH1 or LH2 complexes, respectively, but they are not essential; the few LH1 subunits found in ΔlhaA mutants assemble to form normal RC-LH1-PufX core complexes showing that, once initiated, LH1 assembly round the RC is cooperative and proceeds to completion. LhaA and PucC form oligomers at sites of initiation of membrane invagination; LhaA associates with RCs, bacteriochlorophyll synthase (BchG), the protein translocase subunit YajC and the YidC membrane protein insertase. These associations within membrane nanodomains likely maximise interactions between pigments newly arriving from BchG and nascent proteins within the SecYEG-SecDF-YajC-YidC assembly machinery, thereby co-ordinating pigment delivery, the co-translational insertion of LH polypeptides and their folding and assembly to form photosynthetic complexes.
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