ArticleEpidemiology and infection2015
Determination of cut-off cycle threshold values in routine RT-PCR assays to assist differential diagnosis of norovirus in children hospitalized for acute gastroenteritis.
Article in Epidemiology and infection, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Determination of diagnostic cycle threshold cutoffs for qPCR-based prevalence surveys of soil-transmitted helminth infections.Microbiology spectrum · 2026Trial
- Inferring cell division from cell shape.The Plant cell · 2026Article
- Occurrence of Human Enteric Viruses in Retail Mussels Marketed in Burgos, Spain.Microorganisms · 2026Article
- A report for the fifth International Workshop on Rotavirus and Norovirus in China.Human vaccines & immunotherapeutics · 2025Article
- PPIxGPN: plasma proteomic profiling of neurodegenerative biomarkers with protein-protein interaction-based eXplainable graph propagational network.Briefings in bioinformatics · 2025Article
- Bimodal distributions of anti-Trypanosoma cruzi antibody levels in blood donors are associated with parasite detection and antibody waning in peripheral blood.PLoS neglected tropical diseases · 2025Article
- Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus.Food and environmental virology · 2025Article
- Factors associated with antibiotic use in children hospitalized for acute viral gastroenteritis and the relation to rotavirus vaccination.Human vaccines & immunotherapeutics · 2024Article
- Trends in SARS-CoV-2 Cycle Threshold Values in Bosnia and Herzegovina-A Retrospective Study.Microorganisms · 2024Article
- Semi-quantitative assessment of gastrointestinal viruses in stool samples with Seegene Allplex gastrointestinal panel assays: a solution to the interpretation problem of multiple pathogen detection?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- A bead-based multiplex assay covering all coronaviruses pathogenic for humans for sensitive and specific surveillance of SARS-CoV-2 humoral immunity.Scientific reports · 2023Article
- Trends in SARS-CoV-2 cycle threshold values in the Czech Republic from April 2020 to April 2022.Scientific reports · 2023Article
- Pan-cancer classification of single cells in the tumour microenvironment.Nature communications · 2023Article
- RT-PCR-based assessment of the SD Bioline Rota/Adeno Antigen-based test in infants with and without diarrhea.Virology journal · 2023Article
- Effects of social isolation on 50-kHz ultrasonic vocalizations, affective state, cognition, and neurotransmitter concentrations in the ventral tegmental and locus coeruleus of adult rats.Behavioural brain research · 2023Article
- Cryptic functional diversity within a grass mycobiome.PloS one · 2023Article
- scDemultiplex: An iterative beta-binomial model-based method for accurate demultiplexing with hashtag oligos.Computational and structural biotechnology journal · 2023Article
- Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-β selection thymocytes.Nature communications · 2022Article
- Morphology, physiology and synaptic connectivity of local interneurons in the mouse somatosensory thalamus.The Journal of physiology · 2021Article
- The impact of coronavirus SARS-CoV-2 (COVID-19) in water: potential risks.Environmental science and pollution research international · 2021Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Norovirus (NV) is an important cause of acute gastroenteritis in children, but is also frequently detected in asymptomatic children, which complicates the interpretation of NV detection results in both the clinical setting and population prevalence studies. A total of 807 faecal samples from children aged <5 years hospitalized for acute gastroenteritis were collected in Thai Binh, Vietnam, from January 2011 to September 2012. Real-time RT-PCR was used to detect and quantify NV-RNA in clinical samples. A bimodal distribution of cycle threshold (Ct) values was observed in which the lower peak was assumed to represent cases for which NV was the causal agent of diarrhoea, whereas the higher peak was assumed to represent cases involving an alternative pathogen other than NV. Under these assumptions, we applied finite-mixture modelling to estimate a threshold of Ct <21·36 (95% confidence interval 20·29-22·46) to distinguish NV-positive patients for which NV was the likely cause of diarrhoea. We evaluated the validity of the threshold through comparisons with NV antigen ELISA results, and comparisons of Ct values in patients co-infected with rotavirus. We conclude that the use of an appropriate cut-off value in the interpretation of NV real-time RT-PCR results may improve differential diagnosis of enteric infections, and could contribute to improved estimates of the burden of NV disease.
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