ArticleBiomedical optics express2015
Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Qualitative and quantitative assessment ofBiomedical optics express · 2026Article
- Galvanometer-scanning transient phase microscopy with balanced detection and arbitrary pump polarization.Journal of the Optical Society of America. B, Optical physics · 2026Article
- Article
- Nondestructive, High-Resolution T Cell Characterization and Subtyping via Deep-UV Microscopy.BME frontiers · 2026Article
- Angle-resolved Measurements Reveal the Origin of Signal Anisotropy in Pump-probe Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Label-free in-line characterization of immune cell culture using quantitative phase imaging.NPJ Regenerative medicine · 2025Article
- Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids.Scientific reports · 2024Article
- Non-Invasive Label-free Analysis Pipeline for In Situ Characterization of Differentiation in 3D Brain Organoid Models.Research square · 2024Article
- Recent Advances in Characterization of Melanin Pigments in Biological Samples.International journal of molecular sciences · 2023Review
- Optical characteristics of the skin with dark circles using pump-probe imaging.Scientific reports · 2022Article
- Contrast mechanisms in pump-probe microscopy of melanin.Optics express · 2022Article
- Prostate cancer histopathology using label-free multispectral deep-UV microscopy quantifies phenotypes of tumor aggressiveness and enables multiple diagnostic virtual stains.Scientific reports · 2022Article
- Towards in-vivo label-free detection of brain tumor margins with epi-illumination tomographic quantitative phase imaging.Biomedical optics express · 2021Article
- Multimodal Metabolic Imaging Reveals Pigment Reduction and Lipid Accumulation in Metastatic Melanoma.BME frontiers · 2021Article
- Melanin distribution from the dermal-epidermal junction to the stratum corneum: non-invasive in vivo assessment by fluorescence and Raman microspectroscopy.Scientific reports · 2020Article
- Transient absorption microscopy: Technological innovations and applications in materials science and life science.The Journal of chemical physics · 2020Article
- Analysis of Melanin Structure and Biochemical Composition in Conjunctival Melanocytic Lesions Using Pump-Probe Microscopy.Translational vision science & technology · 2019Article
- Unraveling the molecular nature of melanin changes in metastatic cancer.Journal of biomedical optics · 2019Article
- The changing landscape of dermatology practice: melanoma and pump-probe laser microscopy.Lasers in medical science · 2017Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Metastatic melanoma is associated with a poor prognosis, but no method reliably predicts which melanomas of a given stage will ultimately metastasize and which will not. While sentinel lymph node biopsy (SLNB) has emerged as the most powerful predictor of metastatic disease, the majority of people dying from metastatic melanoma still have a negative SLNB. Here we analyze pump-probe microscopy images of thin biopsy slides of primary melanomas to assess their metastatic potential. Pump-probe microscopy reveals detailed chemical information of melanin with subcellular spatial resolution. Quantification of the molecular signatures without reference standards is achieved using a geometrical representation of principal component analysis. Melanin structure is analyzed in unison with the chemical information by applying principles of mathematical morphology. Results show that melanin in metastatic primary lesions has lower chemical diversity than non-metastatic primary lesions, and contains two distinct phenotypes that are indicative of aggressive disease. Further, the mathematical morphology analysis reveals melanin in metastatic primary lesions has a distinct "dusty" quality. Finally, a statistical analysis shows that the combination of the chemical information with spatial structures predicts metastatic potential with much better sensitivity than SLNB and high specificity, suggesting pump-probe microscopy can be an important tool to help predict the metastatic potential of melanomas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.