ArticleBiomedical optics express2015
Rapid spontaneous Raman light sheet microscopy using cw-lasers and tunable filters.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- High-throughput spontaneous Raman imaging of biological specimens with water background reduction.Biomedical optics express · 2026Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Innovative Imaging Techniques: A Conceptual Exploration of Multi-Modal Raman Light Sheet Microscopy.Micromachines · 2023Article
- Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases.International journal of molecular sciences · 2023Review
- Modular multimodal platform for classical and high throughput light sheet microscopy.Scientific reports · 2022Article
- A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems.RSC chemical biology · 2021Review
- Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications.Communications biology · 2021Article
- Accelerated Stimulated Raman Projection Tomography by Sparse Reconstruction From Sparse-View Data.IEEE transactions on bio-medical engineering · 2020Article
- Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser.Methods and protocols · 2019Article
- Application of Vibrational Spectroscopy and Imaging to Point-of-Care Medicine: A Review.Applied spectroscopy · 2018Review
- Volumetric chemical imaging by stimulated Raman projection microscopy and tomography.Nature communications · 2017Article
- A guide to light-sheet fluorescence microscopy for multiscale imaging.Nature methods · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We perform rapid spontaneous Raman 2D imaging in light-sheet microscopy using continuous wave lasers and interferometric tunable filters. By angularly tuning the filter, the cut-on/off edge transitions are scanned along the excited Stokes wavelengths. This allows obtaining cumulative intensity profiles of the scanned vibrational bands, which are recorded on image stacks; resembling a spectral version of the knife-edge technique to measure intensity profiles. A further differentiation of the stack retrieves the Raman spectra at each pixel of the image which inherits the 3D resolution of the host light sheet system. We demonstrate this technique using solvent solutions and composites of polystyrene beads and lipid droplets immersed in agar and by imaging the C-H (2800-3100cm(-1)) region in a C. elegans worm. The image acquisition time results in 4 orders of magnitude faster than confocal point scanning Raman systems, allowing the possibility of performing fast spontaneous Raman·3D-imaging on biological samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.