ArticleNeuroImage2016
Transcranial functional ultrasound imaging of the brain using microbubble-enhanced ultrasensitive Doppler.
Article in NeuroImage, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Generalizable neural decoding for clinically scalable brain-computer interfaces.npj biomedical innovations · 2026Review
- Mapping blood vessels and oxygenation throughout the whole mouse brain with dual-frequency fiber-array photoacoustic computed tomography.Light, science & applications · 2026Article
- Microbubble Track-Based Functional Ultrasound Localization Microscopy in Awake Mice.IEEE transactions on medical imaging · 2026Article
- Microbubble Backscattering Intensity Improves the Sensitivity of Three-Dimensional (3-D) Functional Ultrasound Localization Microscopy (fULM).IEEE transactions on medical imaging · 2026Article
- Double-helix optical point spread function enables real-time mesoscopic 3D functional microangiography in the living mouse brain and skull.Nature communications · 2026Article
- Microbubble Backscattering Intensity Improves the Sensitivity of Three-dimensional (3D) Functional Ultrasound Localization Microscopy (fULM).bioRxiv : the preprint server for biology · 2025Article
- The non-invasive transcranial Doppler for hemodynamic monitoring.Physiological research · 2025Article
- Human spinal cord activation during filling and emptying of the bladder.Nature communications · 2025Article
- Ultrasound flow imaging for assessing cerebrovascular changes following focused-ultrasound blood-brain barrier opening.Theranostics · 2025Article
- Non-invasive 4D transcranial functional ultrasound and ultrasound localization microscopy for multimodal imaging of neurovascular response.Scientific reports · 2024Article
- Dynamics of cerebral blood volume during and after middle cerebral artery occlusion in rats - Comparison between ultrafast ultrasound and dynamic susceptibility contrast-enhanced MRI measurements.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024Article
- Light on Alzheimer's disease: from basic insights to preclinical studies.Frontiers in aging neuroscience · 2024Review
- Ultrasonic barrier-through imaging by Fabry-Perot resonance-tailoring panel.Nature communications · 2023Article
- Ultrasound contrast agents from microbubbles to biogenic gas vesicles.Medical review (2021) · 2023Review
- The Emergence of Functional Ultrasound for Noninvasive Brain-Computer Interface.Research (Washington, D.C.) · 2023Review
- Retinal functional ultrasound imaging (rfUS) for assessing neurovascular alterations: a pilot study on a rat model of dementia.Scientific reports · 2022Article
- High-resolution micro-Doppler imaging during neurosurgical resection of an arteriovenous malformation: illustrative case.Journal of neurosurgery. Case lessons · 2022Article
- Guiding and monitoring focused ultrasound mediated blood-brain barrier opening in rats using power Doppler imaging and passive acoustic mapping.Scientific reports · 2022Article
- Deep-fUS: A Deep Learning Platform for Functional Ultrasound Imaging of the Brain Using Sparse Data.IEEE transactions on medical imaging · 2022Article
- Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional ultrasound (fUS) is a novel neuroimaging technique, based on high-sensitivity ultrafast Doppler imaging of cerebral blood volume, capable of measuring brain activation and connectivity in rodents with high spatiotemporal resolution (100μm, 1ms). However, the skull attenuates acoustic waves, so fUS in rats currently requires craniotomy or a thinned-skull window. Here we propose a non-invasive approach by enhancing the fUS signal with a contrast agent, inert gas microbubbles. Plane-wave illumination of the brain at high frame rate (500Hz compounded sequence with three tilted plane waves, PRF=1500Hz with a 128 element 15MHz linear transducer), yields highly-resolved neurovascular maps. We compared fUS imaging performance through the intact skull bone (transcranial fUS) versus a thinned-skull window in the same animal. First, we show that the vascular network of the adult rat brain can be imaged transcranially only after a bolus intravenous injection of microbubbles, which leads to a 9dB gain in the contrast-to-tissue ratio. Next, we demonstrate that functional increase in the blood volume of the primary sensory cortex after targeted electrical-evoked stimulations of the sciatic nerve is observable transcranially in presence of contrast agents, with high reproducibility (Pearson's coefficient ρ=0.7±0.1, p=0.85). Our work demonstrates that the combination of ultrafast Doppler imaging and injection of contrast agent allows non-invasive functional brain imaging through the intact skull bone in rats. These results should ease non-invasive longitudinal studies in rodents and open a promising perspective for the adoption of highly resolved fUS approaches for the adult human brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.