ArticleBiotechnology and bioengineering2016
Sphingolipids contribute to acetic acid resistance in Zygosaccharomyces bailii.
Article in Biotechnology and bioengineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- Exploring the interplay between yeast cell membrane lipid adaptation and physiological response to acetic acid stress.Applied and environmental microbiology · 2024Article
- Article
- Shared and more specific genetic determinants and pathways underlying yeast tolerance to acetic, butyric, and octanoic acids.Microbial cell factories · 2024Article
- The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts.FEMS yeast research · 2024Review
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass.Scientific reports · 2023Article
- An Evolved Strain of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids.Microbiology spectrum · 2023Article
- The Influence of Heteroresistance on Minimum Inhibitory Concentration, Investigated Using Weak-Acid Stress in Food Spoilage Yeasts.Applied and environmental microbiology · 2023Article
- Determining small-molecule permeation through lipid membranes.Nature protocols · 2022Review
- Loss of tolerance to multiple environmental stresses due to limitation of structural diversity of complex sphingolipids.Molecular biology of the cell · 2022Article
- Whole-Genome Transformation of Yeast Promotes Rare Host Mutations with a Single Causative SNP Enhancing Acetic Acid Tolerance.Molecular and cellular biology · 2022Article
- Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18.Journal of fungi (Basel, Switzerland) · 2022Article
- The Role of Sch9 and the V-ATPase in the Adaptation Response to Acetic Acid and the Consequences for Growth and Chronological Lifespan.Microorganisms · 2021Article
- Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae.Scientific reports · 2021Article
- Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall.Scientific reports · 2021Article
- The revenge of Zygosaccharomyces yeasts in food biotechnology and applied microbiology.World journal of microbiology & biotechnology · 2021Review
- Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors.Scientific reports · 2021Article
- The Plasma Membrane at the Cornerstone Between Flexibility and Adaptability: Implications forFrontiers in microbiology · 2021Review
- Investigation of lipid profile in Acetobacter pasteurianus Ab3 against acetic acid stress during vinegar production.Extremophiles : life under extreme conditions · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignocellulosic raw material plays a crucial role in the development of sustainable processes for the production of fuels and chemicals. Weak acids such as acetic acid and formic acid are troublesome inhibitors restricting efficient microbial conversion of the biomass to desired products. To improve our understanding of weak acid inhibition and to identify engineering strategies to reduce acetic acid toxicity, the highly acetic-acid-tolerant yeast Zygosaccharomyces bailii was studied. The impact of acetic acid membrane permeability on acetic acid tolerance in Z. bailii was investigated with particular focus on how the previously demonstrated high sphingolipid content in the plasma membrane influences acetic acid tolerance and membrane permeability. Through molecular dynamics simulations, we concluded that membranes with a high content of sphingolipids are thicker and more dense, increasing the free energy barrier for the permeation of acetic acid through the membrane. Z. bailii cultured with the drug myriocin, known to decrease cellular sphingo-lipid levels, exhibited significant growth inhibition in the presence of acetic acid, while growth in medium without acetic acid was unaffected by the myriocin addition. Furthermore, following an acetic acid pulse, the intracellular pH decreased more in myriocin-treated cells than in control cells. This indicates a higher inflow rate of acetic acid and confirms that the reduction in growth of cells cultured with myriocin in the medium with acetic acid was due to an increase in membrane permeability, thereby demonstrating the importance of a high fraction of sphingolipids in the membrane of Z. bailii to facilitate acetic acid resistance; a property potentially transferable to desired production organisms suffering from weak acid stress.
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