Evidence map›Paper›PMID 26416420›Full record

ArticleOncotarget2015

PDGF upregulates CLEC-2 to induce T regulatory cells.

Sudhanshu Agrawal, Sreerupa Ganguly, Pega Hajian, Jia-Ning Cao, Anshu Agrawal

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
1.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 59 citations in OpenAlex.

  1. Trial
  2. Article
  3. Linking tumour angiogenesis and tumour immunity.Nature reviews. Immunology · 2026
    Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. The Binary Classification of Protein Kinases.Journal of inflammation research · 2021
    Article
  18. Article
  19. Article
  20. Podoplanin in Inflammation and Cancer.International journal of molecular sciences · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sudhanshu AgrawalDivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA, USA.
Sreerupa GangulyDivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA, USA.
Pega HajianDivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA, USA.
Jia-Ning CaoDivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA, USA.
Anshu AgrawalDivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA, USA.
University of California, Irvine · US

Funding

Institute for Clinical and Translational ScienceUL1TR000153 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M · 2012 to 2015
$12.1M
Impaired ability of aged human dendritic cells to maintain mucosal toleranceR01AG045216 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI AGRAWAL, ANSHU · 2014 to 2018
$1.6M
NCATS NIH HHS UL1 TR000153NIA NIH HHS AG045216NIA NIH HHS R01 AG045216
6 · The paper itself

Abstract

The effect of platelet derived growth factor (PDGF) on immune cells is not elucidated. Here, we demonstrate PDGF inhibited the maturation of human DCs and induced IL-10 secretion. Culture of PDGF-DCs with T cells induced the polarization of T cells towards FoxP3 expressing T regulatory cells that secreted IL-10. Gene expression studies revealed that PDGF induced the expression of C-type lectin like receptor member 2, (CLEC-2) receptor on DCs. Furthermore, DCs transfected with CLEC-2 induced T regulatory cells in DC-T cell co-culture. CLEC-2 is naturally expressed on platelets. Therefore, to confirm whether CLEC-2 is responsible for inducing the T regulatory cells, T cells were cultured with either CLEC-2 expressing platelets or soluble CLEC-2. Both conditions resulted in the induction of regulatory T cells. The generation of T regulatory cells was probably due to the binding of CLEC-2 with its ligand podoplanin on T cells, since crosslinking of podoplanin on the T cells also resulted in the induction of T regulatory cells. These data demonstrate that PDGF upregulates the expression of CLEC-2 on cells to induce T regulatory cells.

Indexed as

Blood PlateletsCells, CulturedCoculture TechniquesDendritic CellsDose-Response Relationship, DrugForkhead Transcription FactorsHumansInterleukin-10Lectins, C-TypeMembrane GlycoproteinsParacrine CommunicationPhenotypePlatelet-Derived Growth FactorPodoplaninSignal TransductionT-Lymphocytes, RegulatoryCLEC2B protein, humanForkhead Transcription FactorsFOXP3 protein, humanIL10 protein, humanInterleukin-10Lectins, C-TypeMembrane GlycoproteinsPDPN protein, humanPlatelet-Derived Growth Factorplatelet-derived growth factor ABPodoplaninClec-2dendritic cellImmune responseImmunityImmunology and Microbiology SectionPDGFplateletspodoplaninT regulatory cells

Identifiers

PMID26416420
PMCPMC4745681
OpenAlexW1959403697

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.