Evidence map›Paper›PMID 26416350›Full record

ArticleTissue engineering. Part A2015

The Effects of Platelet-Rich Plasma on Cell Proliferation and Adipogenic Potential of Adipose-Derived Stem Cells.

Han Tsung Liao, Isaac B James, Kacey G Marra, J Peter Rubin

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Article in Tissue engineering. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
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  15. Therapeutic options for premature ovarian insufficiency: an updated review.Reproductive biology and endocrinology : RB&E · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Han Tsung Liao1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania .
Isaac B James1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania .
Kacey G Marra1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania .
J Peter Rubin1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania .

Funding

Injectable Engineered Tissue for Cancer ReconstructionR01CA114246 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI RUBIN, J PETER · 2006 to 2019
$3.3M
NCI NIH HHS R01 CA114246NCI NIH HHS R01-CA114246
6 · The paper itself

Abstract

backgroundPlatelet-rich plasma (PRP) contains multiple growth factors and has been shown to enhance fat graft survival after lipotransfer. However, the molecular mechanisms mediating this effect remain unknown. Adipose-derived stem cells (ASCs) play an important role in fat graft survival and are a likely target for PRP-mediated effects. This study seeks to investigate the impact of PRP on ASC proliferation and adipogenic differentiation.

methodsHuman ASCs were isolated using our laboratory protocol. The experiments were divided into four arms: (1) ASCs cultured in general culture medium alone; (2) ASCs in general culture medium + 5%, 10%, 15%, or 20% PRP; (3) ASCs cultured in adipogenic differentiation medium alone; (4) ASCs cultured in adipogenic medium + 5%, 10%, 15%, or 20% PRP. Cell proliferation was analyzed and comparative m-RNA expression of adipogenic genes was assessed by quantitative PCR. Protein expression was determined by western blot.

resultsPRP significantly enhanced proliferation of ASCs, even in the presence of antiproliferative, proadipogenic media. In contrast, PRP inhibited adipogenic differentiation in adipogenic media, evidenced by decreased intracellular lipid accumulation and reduced adipogenic gene expression (PPAR-γ and FABP4). Inhibition appears to occur through downregulation of bone morphogenetic protein receptor IA (BMPRIA) and fibroblast growth factor receptor 1 (FGFR1). Interestingly, PRP elicited these effects across the entire range of doses studied.

conclusionsPRP appears to modulate ASC function primarily by enhancing cell proliferation. The consequences of its impact on adipogenesis are less clear. Enhanced proliferation initially might set the stage for more robust regeneration and adipogenesis at later time points, providing an important target for ongoing research.

Indexed as

AdipocytesAdipogenesisBatch Cell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedHumansPlatelet-Rich PlasmaStem Cells

Identifiers

PMID26416350
PMCPMC4652209

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.