Evidence map›Paper›PMID 26415963›Full record

ArticleWorld journal of microbiology & biotechnology2015

Cell culture on hydrophilicity-controlled silicon nitride surfaces.

Yuriko Masuda, Wataru Inami, Atsuo Miyakawa, Yoshimasa Kawata

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuriko MasudaShizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan.
Wataru InamiShizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan.
Atsuo MiyakawaShizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan.
Yoshimasa KawataShizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan. kawata@eng.shizuoka.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell culture on silicon nitride membranes is required for atmospheric scanning electron microscopy, electron beam excitation assisted optical microscopy, and various biological sensors. Cell adhesion to silicon nitride membranes is typically weak, and cell proliferation is limited. We increased the adhesion force and proliferation of cultured HeLa cells by controlling the surface hydrophilicity of silicon nitride membranes. We covalently coupled carboxyl groups on silicon nitride membranes, and measured the contact angles of water droplets on the surfaces to evaluate the hydrophilicity. We cultured HeLa cells on the coated membranes and evaluated stretch of the cell. Cell migration and confluence were observed on the coated silicon nitride films. We also demonstrated preliminary observation result with direct electron beam excitation-assisted optical microscope.

Indexed as

Hydrophobic and Hydrophilic InteractionsBiocompatible MaterialsCell AdhesionCell Culture TechniquesCell ProliferationFocal AdhesionsHeLa CellsHumansMembranesMicroscopy, Electron, ScanningSilicon CompoundsSurface PropertiesBiocompatible MaterialsSilicon Compoundssilicon nitrideCarboxyl groupCell adhesionHeLa cellSilicon nitrideSurface modification

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.