Evidence map›Paper›PMID 26415609›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Isolation of Circulating Melanoma Cells.

Jie Ma, Markus H Frank

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jie MaDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Markus H FrankDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. markus.frank@childrens.harvard.edu.
Brigham and Women's Hospital · US

Funding

Targeting Raf in Melanoma: Mechanisms for Effect & Resistance & Opportunities forP50CA093683 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI GOLUB, TODD R. · 2001 to 2012
$25.8M
DERMATOLOGY TRAINING GRANTT32AR007098 · NIAMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI THOMAS S. KUPPER · 1986 to 2026
$8.1M
Immunology of human malignant melanoma initiating cellsR01CA158467 · NCI · BOSTON CHILDREN'S HOSPITAL · PI FRANK, MARKUS H., HODI, FRANK S · 2012 to 2016
$1.9M
Melanoma Stem Cells, Vasculogenesis and Neoplastic ProgressionR01CA138231 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI FRANK, MARKUS H. · 2008 to 2012
$1.7M
ABCB5 P-Glycoprotein in Cancer Multidrug ResistanceR01CA113796 · NCI · BOSTON CHILDREN'S HOSPITAL · PI FRANK, MARKUS H. · 2006 to 2010
$1.5M
NCI NIH HHS P50 CA093683NCI NIH HHS R01 CA113796NCI NIH HHS R01 CA138231NCI NIH HHS R01 CA158467NIAMS NIH HHS T32 AR007098
6 · The paper itself

Abstract

Circulating melanoma cells (CMCs) represent critical mediators of metastatic melanoma progression. However, isolation and characterization of CMCs has been challenging due to the low frequency of these cells and the paucity of melanoma-specific cell surface markers. Herein, we describe a method for the isolation of CMCs that employs two independent markers, displays high sensitivity for CMC enrichment, and can be readily adapted to include additional molecular melanoma markers of interest. CMCs isolated by this method are enriched for ABCB5-positive melanoma stem cells, are tumorigenic in xenotransplantation assays, and can be used for phenotypical, genetic, and functional investigations of CMC biology.

Indexed as

ABCB5Circulating melanoma cellsCirculating tumor cellsFlow cytometryMelanoma stem cellsMetastasisTumorigenicityXenotransplantation

Identifiers

PMID26415609
PMCPMC4884170
OpenAlexW2417398125

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.