Evidence map›Paper›PMID 26413786›Full record

ArticleStem cell research2015

Establishment of leukemia inhibitory factor (LIF)-independent iPS cells with potentiated Oct4.

Hiroyuki Hirai, Meri Firpo, Nobuaki Kikyo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Hiroyuki HiraiStem Cell Institute, University of Minnesota, Minneapolis, Minnesota 55455, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Meri FirpoStem Cell Institute, University of Minnesota, Minneapolis, Minnesota 55455, USA; Division of Endocrinology, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Nobuaki KikyoStem Cell Institute, University of Minnesota, Minneapolis, Minnesota 55455, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA. Electronic address: kikyo001@umn.edu.
University of Minnesota · US

Funding

Women's CancerP30CA077598 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI HEATHER Hammond NELSON · 1998 to 2026
$100.4M
Histone isomerization and pluripotencyR01GM098294 · NIGMS · UNIVERSITY OF MINNESOTA · PI KIKYO, NOBUAKI · 2012 to 2015
$1.1M
Hypoxia and long noncoding RNAR21CA187232 · NCI · UNIVERSITY OF MINNESOTA · PI KIKYO, NOBUAKI · 2014 to 2015
$364k
NCI NIH HHS P30 CA077598NCI NIH HHS R21 CA187232NIGMS NIH HHS R01 GM098294
6 · The paper itself

Abstract

Leukemia inhibitory factor (LIF) is widely used to establish and maintain naïve pluripotent stem cells, including mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Although the combination of chemical inhibitors called 2i can establish mouse iPSCs without LIF from primed pluripotent stem cells, it has been difficult, if not impossible, to establish mouse iPSCs from differentiated somatic cells without LIF. We previously showed that the fusion gene of the transactivation domain of MyoD and the full-length Oct4 (M3O) increases the efficiency of making iPSCs when transduced into fibroblasts along with Sox2, Klf4, and c-Myc (M3O-SKM). Here, we report that M3O-SKM allows for establishment of iPSCs without exogenous LIF from mouse embryonic fibroblasts. The established iPSCs remained undifferentiated and maintained pluripotency over 90 days without LIF as long as M3O was expressed. The iPSCs upregulated miR-205-5p, which was potentially involved in the LIF-independence by suppressing the two signaling pathways inhibited by 2i. The result indicates that potentiated Oct4 can substitute for the LIF signaling pathway, providing a novel model to link Oct4 and LIF, two of the most significant players in naïve pluripotency.

Indexed as

AnimalsCell DifferentiationInduced Pluripotent Stem CellsKruppel-Like Factor 4Leukemia Inhibitory FactorMiceMice, TransgenicMicroRNAsOctamer Transcription Factor-3Signal TransductionTransfectionKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Leukemia Inhibitory FactorMicroRNAsOctamer Transcription Factor-3POU5F1 protein, humanInduced pluripotent stem cellsLeukemia inhibitory factormiRNAPluripotency

Identifiers

PMID26413786
PMCPMC4698063
OpenAlexW1711027972

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.