Evidence map›Paper›PMID 26413302›Full record

ArticleJournal of materials chemistry. B2015

Glutamine-chitosan modified calcium phosphate nanoparticles for efficient siRNA delivery and osteogenic differentiation.

Bogyu Choi, Zhong-Kai Cui, Soyon Kim, Jiabing Fan, Benjamin M Wu, Min Lee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of materials chemistry. B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
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  9. Rational design of hydrogels to enhance osteogenic potential.Chemistry of materials : a publication of the American Chemical Society · 2020
    Article
  10. Review
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  12. Nanostructured Materials for Artificial Tissue Replacements.International journal of molecular sciences · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Bogyu ChoiDivision of Advanced Prosthodontics, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, California 90095, USA.
Zhong-Kai CuiDivision of Advanced Prosthodontics, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, California 90095, USA.
Soyon KimDepartment of Bioengineering, University of California Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, USA.
Jiabing FanDivision of Advanced Prosthodontics, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, California 90095, USA.
Benjamin M WuDivision of Advanced Prosthodontics, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, California 90095, USA ; Department of Bioengineering, University of California Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, USA.
Min LeeDivision of Advanced Prosthodontics, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, California 90095, USA ; Department of Bioengineering, University of California Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, USA.
Los Angeles City College · USUniversity of California, Los Angeles · US

Funding

Combined Effect of Noggin Suppression and Nell-1 on Bone RegenerationR01AR060213 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LEE, MIN · 2011 to 2015
$1.7M
Biomimetic Scaffold Delivering Osteogenic Molecules for Alveolar Bone EngineeringR21DE021819 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LEE, MIN · 2012 to 2013
$416k
NIAMS NIH HHS R01 AR060213NIDCR NIH HHS R21 DE021819
6 · The paper itself

Abstract

RNA interference (RNAi)-based therapy using small interfering RNA (siRNA) exhibits great potential to treat diseases. Although calcium phosphate (CaP)-based systems are attractive options to deliver nucleic acids due to their good biocompatibility and high affinity with nucleic acids, they are limited by uncontrollable particle formation and inconsistent transfection efficiencies. In this study, we developed a stable CaP nanocarrier system with enhanced intracellular uptake by adding highly cationic, glutamine-conjugated oligochitosan (Gln-OChi). CaP nanoparticles coated with Gln-OChi (CaP/Gln-OChi) significantly enhanced gene transfection and knockdown efficiency in both immortalized cell line (HeLa) and primary mesenchymal stem cells (MSCs) with minimal cytotoxicity. The osteogenic bioactivity of siRNA-loaded CaP/Gln-OChi particles was further confirmed in three-dimensional environments by using photocrosslinkable chitosan hydrogels encapsulating MSCs and particles loaded with siRNA targeting noggin, a bone morphogenetic protein antagonist. These findings suggest that our CaP/Gln-OChi nanocarrier provides an efficient and safe gene delivery system for therapeutic applications.

Identifiers

PMID26413302
PMCPMC4582691
OpenAlexW2098220543

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.