ArticleNature communications2015
Optodynamic simulation of β-adrenergic receptor signalling.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed, 104 citations in OpenAlex.
- β-Adrenergic receptors as immunomodulators in T cells: mechanisms of neuroimmune crosstalk and pathophysiological implications.Molecular medicine (Cambridge, Mass.) · 2026Review
- Light-Based Molecular Tools to Precisely Monitor and Operate β-Adrenoceptors.Medicinal research reviews · 2026Review
- Interrogating Physiological Functions with Light and Chemicals.Annual review of physiology · 2026Review
- Optogenetic engineering of synthetic and natural receptors: design principles, functional mechanisms and biomedical applications.Regenerative biomaterials · 2026Review
- Recent Developments in the Optical Control of Adrenergic Signaling.Medicinal research reviews · 2025Review
- Identification of a stress-responsive subregion of the basolateral amygdala in male rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- Neuromodulator and neuropeptide sensors and probes for precise circuit interrogation in vivo.Science (New York, N.Y.) · 2024Review
- A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits.Nature methods · 2024Article
- Protein-Based Tools for Studying Neuromodulation.ACS chemical biology · 2024Review
- Targeting Histamine and Histamine Receptors for Memory Regulation: An Emotional Perspective.Current neuropharmacology · 2024Review
- Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors.Nature communications · 2023Article
- Optical Approaches for Investigating Neuromodulation and G Protein-Coupled Receptor Signaling.Pharmacological reviews · 2023Review
- Astrocytes: new evidence, new models, new roles.Biophysical reviews · 2023Review
- Neuronal and astrocytic CB1R signaling differentially modulates goal-directed behavior and working memory by distinct temporal mechanisms.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells.eLife · 2023Article
- A bistable inhibitory OptoGPCR for multiplexed optogenetic control of neural circuits.bioRxiv : the preprint server for biology · 2023Article
- A self-inactivating invertebrate opsin optically drives biased signaling toward Gβγ-dependent ion channel modulation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Functional optimization of light-activatable Opto-GPCRs: Illuminating the importance of the proximal C-terminus in G-protein specificity.Frontiers in cell and developmental biology · 2023Article
- Emerging approaches for decoding neuropeptide transmission.Trends in neurosciences · 2022Review
- Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses.Nature communications · 2022Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Optogenetics has provided a revolutionary approach to dissecting biological phenomena. However, the generation and use of optically active GPCRs in these contexts is limited and it is unclear how well an opsin-chimera GPCR might mimic endogenous receptor activity. Here we show that a chimeric rhodopsin/β2 adrenergic receptor (opto-β2AR) is similar in dynamics to endogenous β2AR in terms of: cAMP generation, MAP kinase activation and receptor internalization. In addition, we develop and characterize a novel toolset of optically active, functionally selective GPCRs that can bias intracellular signalling cascades towards either G-protein or arrestin-mediated cAMP and MAP kinase pathways. Finally, we show how photoactivation of opto-β2AR in vivo modulates neuronal activity and induces anxiety-like behavioural states in both fiber-tethered and wireless, freely moving animals when expressed in brain regions known to contain β2ARs. These new GPCR approaches enhance the utility of optogenetics and allow for discrete spatiotemporal control of GPCR signalling in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.