ArticleParasitology research2016
Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods.
Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Prevalence of Free-Living Amoebae in Various Water Sources in Iran: A Systematic Review and Meta-Analysis.Acta parasitologica · 2025Pooled it
- Prevalence of free-living amoebae in swimming pools and recreational waters, a systematic review and meta-analysis.Parasitology research · 2022Pooled it
- Molecular identification of potentially pathogenic free-living amoebae in environmental samples from urban areas of Kerman, Southeastern Iran.Parasite epidemiology and control · 2026Article
- High Diversity and Prevalence of Potentially Pathogenic Free-Living Amoebae in Water Sources from Castilla y León, Spain.Pathogens (Basel, Switzerland) · 2025Article
- Genotyping ofInternational journal of microbiology · 2025Article
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
- Distribution and Current State of Molecular Genetic Characterization in Pathogenic Free-Living Amoebae.Pathogens (Basel, Switzerland) · 2022Review
- Cooccurrence of Five PathogenicPathogens (Basel, Switzerland) · 2021Article
- Article
- Fungal and parasitic contamination of indoor public swimming pools in Arak, Iran.The Journal of the Egyptian Public Health Association · 2020Article
- Isolation and Genotyping of Acanthamoeba from Soil Samples in Markazi Province, Iran.Open access Macedonian journal of medical sciences · 2018Article
- Vermamoeba vermiformis: a Free-Living Amoeba of Interest.Microbial ecology · 2018Review
- Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.BMC microbiology · 2018Article
- Characterization of extracellular proteases of Acanthamoeba genotype T4 isolated from different sources in Iran.Parasitology research · 2017Article
- Occurrence of Infected Free-Living Amoebae in Cooling Towers of Southern Brazil.Current microbiology · 2017Article
- Free-living amoebae in the water resources of Iran: a systematic review.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Review
- Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny.Parasitology research · 2017Article
- Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells.Parasitology research · 2016Article
- Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt.Parasitology research · 2016Article
- Occurrence ofIranian journal of parasitologyReview
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae (FLA) are the most abundant and widely distributed protozoa in the environment. An investigation was conducted to determine the presence of free-living amoebae (FLA), Acanthamoeba and Vermamoeba in waterfronts of parks and squares and tap water of Shiraz City, Iran. FLA are considered pathogenic for human. These ubiquitous organisms have been isolated from different environments such as water, soil, and air. Eighty-two water samples were collected from different places of Shiraz City during the summer of 2013. All samples were processed in Dept. of Parasitology and Mycology, Shiraz University of Medical Sciences, Fars, Iran. Samples were screened for FLA and identified by morphological characters in the cultures, PCR amplification targeting specific genes for each genus and sequencing determined frequent species and genotypes base on NCBI database. Overall, 48 samples were positive for Acanthamoeba and Vermamoeba in non-nutrient agar culture based on morphological characteristics. The PCR examination was done successfully. Sequencing results were revealed T4 (62.96 %) genotypes as the most common genotype of Acanthamoeba in the Shiraz water sources. In addition, T5 (33.33 %) and T15 (3.71 %) were isolated from water supplies. Vermamoeba vermiformis was known the dominant species from this genus. The high frequency of Acanthamoeba spp. and Vermamoeba in different environmental water sources of Shiraz is an alert for the public health related to water sources. The result highlights a need for taking more attention to water supplies in order to prevent illnesses related to free-living amoebae.
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